Electron Transfer Dissociation (ETD): The Mass Spectrometric Breakthrough Essential for O-GlcNAc Protein Site Assignments-A Study of the O-GlcNAcylated Protein Host Cell Factor C1 (2013)
Samuel A Myers, Salima Daou, El Bachir Affar, Al Burlingame
Proteomics, 2013 vol.13(6):982-91
SNFG,
Text,
Oxford
taxonomy (1)
protein (1)
source (1)
structure (1)
composition (1)
disease (0)
reference (1)
site (18)
peptide (13)
- Homo sapiens (Human)
Taxonomy
- HEK293 (CVCL_0045)
Source
- O-Linked / O-GlcNAc / GlcNAc(b
Reported structure
- HexNAc:1 (avg mass : 221.2103 )
Composition
Disease
- Host Cell Factor 1 / Homo sapiens
Reported glycosite
- VTGPQATGTPLVTMRPASQAGK (22aa)
- IPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVK (36aa)
- TAAAQVGTSVSSATNTSTRPIITVHK (26aa)
- SGTVTVAQQAQVVTTVVGGVTK (22aa)
- VMSVVQTKPVQTSAVTGQASTGPVTQIIQTK (31aa)
- LVTSADGK (8aa)
- TIPMSAIITQAGATGVTSSPGIK (23aa)
- LVTPVTVSAVKPAVTTLVVK (20aa)
- TGTTNTATTTVVANLGGHPQPTQVQFVCDRQE (32aa)
- RACAAGTPAVIR (12aa)
- SLQGGSPSTTVTVTALE (17aa)
- TGTTHTATTATSNGGTGQPE (20aa)
- LAGTVPSTVALLPSTATE (18aa)
Mass spectrometry observed peptide
-
- O-Linked / O-GlcNAc
(avg mass : 221.2103)
- HEK293 (CVCL_0045)
- Host Cell Factor 1 / Homo sapiens
- VTGPQATGTPLVTMRPASQAGK (22aa)
- IPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVK (36aa)
- TAAAQVGTSVSSATNTSTRPIITVHK (26aa)
- SGTVTVAQQAQVVTTVVGGVTK (22aa)
- VMSVVQTKPVQTSAVTGQASTGPVTQIIQTK (31aa)
- LVTSADGK (8aa)
- TIPMSAIITQAGATGVTSSPGIK (23aa)
- LVTPVTVSAVKPAVTTLVVK (20aa)
- TGTTNTATTTVVANLGGHPQPTQVQFVCDRQE (32aa)
- RACAAGTPAVIR (12aa)
- SLQGGSPSTTVTVTALE (17aa)
- TGTTHTATTATSNGGTGQPE (20aa)
- LAGTVPSTVALLPSTATE (18aa)
Source
Reported glycosite
Mass spectrometry observed peptide
- O-Linked / O-GlcNAc
(avg mass : 221.2103)
