Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer

Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• Schneider 2 (CVCL_Z232)  

Reported structure

• O-Linked / Core 0 / GalNAc

Composition

• HexNAc:1 (avg mass : 221.2103 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed

Reported glycosite

• Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-315
  •        Thr-323
  •        Thr-333
  •        Thr-345
  •        Thr-523

Mass spectrometry observed peptide

• TVEKGIYQTSNF (12aa)
  •    P0DTC2    Thr-315     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVR (10aa)
  •    P0DTC2    Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVRFPNITNLCPF (20aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• GIYQTSNFRVQPTESIVR (18aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVRFPNITNL (17aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• GEVFNATRFASVY (13aa)
  •    P0DTC2    Thr-345     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NATRFASVY (9aa)
  •    P0DTC2    Thr-345     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAPATVCGPKKSTNL (15aa)
  •    P0DTC2    Thr-523     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)