Cell line HEK293

Taxonomy

• Homo sapiens (Human)

Protein

• Histone H3 / Homo sapiens    P68431
• Host Cell Factor 1 / Homo sapiens    P51610

Source

• HEK293 (CVCL_0045)  

Reported structure

• O-Linked / O-GlcNAc / GlcNAc(b

Composition

• HexNAc:1 (avg mass : 221.2103 )

Disease

Reference

• Electron Transfer Dissociation (ETD): The Mass Spectrometric Breakthrough Essential for O-GlcNAc Protein Site Assignments-A Study of the O-GlcNAcylated Protein Host Cell Factor C1 (2013 - Samuel A Myers, Salima Daou, El Bachir Affar, Al Burlingame) / Status : Reviewed
• Modification of Histones by Sugar β-N-acetylglucosamine (GlcNAc) Occurs on Multiple Residues, Including Histone H3 Serine 10, and Is Cell Cycle-Regulated (2011 - Suisheng Zhang, Kevin Roche, Heinz-Peter Nasheuer, Noel Francis Lowndes) / Status : Reviewed

Reported glycosite

• Histone H3 / Homo sapiens
  •        Ser-11
• Host Cell Factor 1 / Homo sapiens
  •        Thr-495
  •        Ser-569
  •        Ser-620
  •        Ser-622
  •        Ser-623
  •        Thr-625
  •        Thr-651
  •        Thr-652
  •        Ser-685
  •        Thr-726
  •        Thr-779
  •        Thr-861
  •        Thr-1030
  •        Thr-1031
  •        Thr-1143
  •        Thr-1273
  •        Thr-1335
  •        Thr-1743

Mass spectrometry observed peptide

• QTARKSTGGKAP (12aa)
  •    P68431    Ser-11     Histone H3 / Homo sapiens
• VTGPQATGTPLVTMRPASQAGK (22aa)
  •    P51610    Thr-495     Host Cell Factor 1 / Homo sapiens
• IPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVK (36aa)
  •    P51610    Ser-569     Host Cell Factor 1 / Homo sapiens
• TAAAQVGTSVSSATNTSTRPIITVHK (26aa)
  •    P51610    Ser-622     Host Cell Factor 1 / Homo sapiens
  •    P51610    Ser-620     Host Cell Factor 1 / Homo sapiens
  •    P51610    Ser-623     Host Cell Factor 1 / Homo sapiens
  •    P51610    Thr-625     Host Cell Factor 1 / Homo sapiens
• SGTVTVAQQAQVVTTVVGGVTK (22aa)
  •    P51610    Thr-651     Host Cell Factor 1 / Homo sapiens
  •    P51610    Thr-652     Host Cell Factor 1 / Homo sapiens
• VMSVVQTKPVQTSAVTGQASTGPVTQIIQTK (31aa)
  •    P51610    Ser-685     Host Cell Factor 1 / Homo sapiens
• LVTSADGK (8aa)
  •    P51610    Thr-726     Host Cell Factor 1 / Homo sapiens
• TIPMSAIITQAGATGVTSSPGIK (23aa)
  •    P51610    Thr-779     Host Cell Factor 1 / Homo sapiens
• LVTPVTVSAVKPAVTTLVVK (20aa)
  •    P51610    Thr-861     Host Cell Factor 1 / Homo sapiens
• TGTTNTATTTVVANLGGHPQPTQVQFVCDRQE (32aa)
  •    P51610    Thr-1030     Host Cell Factor 1 / Homo sapiens
  •    P51610    Thr-1031     Host Cell Factor 1 / Homo sapiens
• RACAAGTPAVIR (12aa)
  •    P51610    Thr-1143     Host Cell Factor 1 / Homo sapiens
• SLQGGSPSTTVTVTALE (17aa)
  •    P51610    Thr-1273     Host Cell Factor 1 / Homo sapiens
• TGTTHTATTATSNGGTGQPE (20aa)
  •    P51610    Thr-1335     Host Cell Factor 1 / Homo sapiens
• LAGTVPSTVALLPSTATE (18aa)
  •    P51610    Thr-1743     Host Cell Factor 1 / Homo sapiens