Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• HEK293-F (CVCL_6642)  
• Schneider 2 (CVCL_Z232)  

Reported structure

• O-Linked / Core 0 / GalNAc

Composition

• HexNAc:1 (avg mass : 221.2103 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed

Reported glycosite

• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-63
  •        Thr-73
  •        Thr-76
  •        Thr-114
  •        Thr-167
  •        Thr-236
  •        Ser-803
  •        Thr-1076
  •        Thr-1077
  •        Ser-1097
• Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Undefined site
  •        Ser-71
  •        Ser-71
  •        Thr-73
  •        Thr-76
  •        Ser-94
  •        Thr-95
  •        Thr-124
  •        Ser-151
  •        Thr-678
  •        Ser-803
  •        Thr-1076
  •        Thr-1077
  •        Ser-1097
• Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-315
  •        Thr-323
  •        Thr-333
  •        Thr-345
  •        Thr-523

Mass spectrometry observed peptide

• FSNVTWFHAIHVSGTNGTK (19aa)
  •    P0DTC2        Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-63     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• FHAIHVSGTNGTKRF (15aa)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAIHVSGTNGTK (12aa)
  •    P0DTC2    Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAIHVSGTNGTKRF (14aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VTWFHAIHVSGTNGTK (16aa)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VSGTNGTKRF (10aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• ASTEKSNIIRGW (12aa)
  •    P0DTC2    Ser-94     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-95     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• TQSLLIVNNATNVVIK (16aa)
  •    P0DTC2    Thr-114     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• IVNNATNVVIKVCEF (15aa)
  •    P0DTC2    Thr-124     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• YHKNNKSWMESEF (13aa)
  •    P0DTC2    Ser-151     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HKNNKSWMESEF (12aa)
  •    P0DTC2    Ser-151     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VYSSANNCTFEYVSQPFLMDLEGK (24aa)
  •    P0DTC2    Thr-167     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• SALEPLVDLPIGINIT (16aa)
  •    P0DTC2    Thr-236     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VDLPIGINIT (10aa)
  •    P0DTC2    Thr-236     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• TVEKGIYQTSNF (12aa)
  •    P0DTC2    Thr-315     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVR (10aa)
  •    P0DTC2    Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVRFPNITNL (17aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVRFPNITNLCPF (20aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• GIYQTSNFRVQPTESIVR (18aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• GEVFNATRFASVY (13aa)
  •    P0DTC2    Thr-345     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NATRFASVY (9aa)
  •    P0DTC2    Thr-345     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAPATVCGPKKSTNL (15aa)
  •    P0DTC2    Thr-523     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• CDIPIGAGICASYQTQTNSPAR (22aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→ARAA
• QTQTNSPARAA (11aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→ARAA
• FGGFNFSQILPDPSKPSKR (19aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NFSQILPDPSKPS (13aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NFSQILPDPSKPSKR (15aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• FGGFNFSQILPDPSKPSK (18aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NFSQILPDPSKPSKRSF (17aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• DFGGFNFSQILPDPSK (16aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• KNFTTAPAICHDGK (14aa)
  •    P0DTC2    Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• AQEKNFTTAPAICHDGKAHFPREGVF (26aa)
  •    P0DTC2    Thr-1077     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1076     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• GVFVSNGTHWFVTQR (15aa)
  •    P0DTC2    Ser-1097     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• EGVFVSNGTHW (11aa)
  •    P0DTC2    Ser-1097     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• EGVFVSNGTHWFVTQR (16aa)
  •    P0DTC2    Ser-1097     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)