Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• HEK293-F (CVCL_6642)  

Reported structure

• O-Linked / Core 1 / Gal(b1-3)GalNAc

Composition

• Hex:1 HexNAc:1 (avg mass : 383.3527 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed

Reported glycosite

• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-676

Mass spectrometry observed peptide

• QTQTNSPGSASSVASQ (16aa)
  •    P0DTC2    Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
• QTQTNSPGSASSVASQSIIAY (21aa)
  •    P0DTC2    Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS