Heterogeneity of the complex N-linked oligosaccharides at specific glycosylation sites of two secreted carrot glycoproteins. (1991)

Taxonomy

• Daucus carota (Carrot)

Protein

• Beta-fructofuranosidase, insoluble isoenzyme 1 / Daucus carota    P26792
• Uncharacterized protein from Culture Medium / Daucus carota    

Source

• Cell Wall (GO_0005618)  

Reported structure

• N-Linked / Complex / GlcNAc(b1-?)Man(a1-?)[Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / Ara(?1-?)[GlcNAc(b1-?)]Man(a1-?)[Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / Ara(?1-?)Man(a1-?)[GlcNAc(b1-?)Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / GlcNAc(b1-?)[Rha(?1-?)]Man(a1-?)[Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / GlcNAc(b1-?)Man(a1-?)[Rha(?1-?)Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / GlcNAc(b1-?)Man(a1-3)[GlcNAc(b1-?)Man(a1-6)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / Ara(?1-?)[GlcNAc(b1-?)]Man(a1-?)[Ara(?1-?)Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / Ara(?1-?)[GlcNAc(b1-?)]Man(a1-?)[Rha(?1-?)Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / GlcNAc(b1-?)[Rha(?1-?)]Man(a1-?)[Ara(?1-?)Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / GlcNAc(b1-?)[Rha(?1-?)]Man(a1-?)[Rha(?1-?)Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / Ara(?1-?)[GlcNAc(b1-?)]Man(a1-?)[GlcNAc(b1-?)Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / GlcNAc(b1-?)[Rha(?1-?)]Man(a1-?)[GlcNAc(b1-?)Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / Ara(?1-?)[GlcNAc(b1-?)]Man(a1-3)[Ara(?1-?)[GlcNAc(b1-?)]Man(a1-6)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / Ara(?1-?)[GlcNAc(b1-?)]Man(a1-?)[GlcNAc(b1-?)[Rha(?1-?)]Man(a1-?)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / Complex / GlcNAc(b1-?)[Rha(?1-?)]Man(a1-3)[GlcNAc(b1-?)[Rha(?1-?)]Man(a1-6)][Xyl(?1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc
• N-Linked / High-Mannose / Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)[Man(a1-2)Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)][Xyl(b1-2)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-3)]GlcNAc

Composition

• Hex:3 HexNAc:2 dHex:1 Pent:1 (avg mass : 1189.0916 )
• Hex:3 HexNAc:3 dHex:1 Pent:1 (avg mass : 1392.2866 )
• Hex:3 HexNAc:3 dHex:1 Pent:2 (avg mass : 1524.4027 )
• Hex:3 HexNAc:3 dHex:1 Pent:3 (avg mass : 1656.5188 )
• Hex:3 HexNAc:3 dHex:2 Pent:1 (avg mass : 1538.4296 )
• Hex:3 HexNAc:3 dHex:2 Pent:2 (avg mass : 1670.5457 )
• Hex:3 HexNAc:3 dHex:3 Pent:1 (avg mass : 1684.5726 )
• Hex:3 HexNAc:4 dHex:1 Pent:1 (avg mass : 1595.4816 )
• Hex:3 HexNAc:4 dHex:1 Pent:2 (avg mass : 1727.5977 )
• Hex:3 HexNAc:4 dHex:1 Pent:3 (avg mass : 1859.7138 )
• Hex:3 HexNAc:4 dHex:2 Pent:1 (avg mass : 1741.6246 )
• Hex:3 HexNAc:4 dHex:2 Pent:2 (avg mass : 1873.7407 )
• Hex:3 HexNAc:4 dHex:3 Pent:1 (avg mass : 1887.7676 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )

Disease

Reference

• Heterogeneity of the complex N-linked oligosaccharides at specific glycosylation sites of two secreted carrot glycoproteins. (1991 - Sturm A) / Status : Reviewed

Reported glycosite

• Beta-fructofuranosidase, insoluble isoenzyme 1 / Daucus carota
  •        Asn-170
  •        Asn-311
  •        Asn-348
• Uncharacterized protein from Culture Medium / Daucus carota
  •        Undefined site

Mass spectrometry observed peptide