Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris (2001)

Taxonomy

• Bos taurus (Bovine)

Protein

• Beta-lactoglobulin / Bos taurus    P02754

Source

Reported structure

• N-Linked / High-Mannose / Man(a1-2)Man(a1-2)[Man(a1-6)]Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Man(a1-2)Man(a1-2)[Man(a1-6)]Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ Man(a1-2)"
• N-Linked / High-Mannose / Man(a1-2)Man(a1-2)[Man(a1-6)]Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ 2 x Man(a1-2)"
• N-Linked / High-Mannose / Man(a1-2)Man(a1-2)[Man(a1-6)]Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ 3 x Man(a1-2)"
• N-Linked / High-Mannose / Man(a1-2)Man(a1-2)[Man(a1-6)]Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ 4 x Man(a1-2)"
• N-Linked / High-Mannose / Man(a1-2)Man(a1-2)[Man(a1-6)]Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ 5 x Man(a1-2)"

Composition

• Hex:9 HexNAc:2 (avg mass : 1883.6869 )
• Hex:10 HexNAc:2 (avg mass : 2045.8293 )
• Hex:11 HexNAc:2 (avg mass : 2207.9717 )
• Hex:12 HexNAc:2 (avg mass : 2370.1141 )
• Hex:13 HexNAc:2 (avg mass : 2532.2565 )
• Hex:14 HexNAc:2 (avg mass : 2694.3989 )

Disease

Reference

• Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris (2001 - Kalidas, Joshi, Batt) / Status : Reviewed

Reported glycosite

• Beta-lactoglobulin / Bos taurus
  •        Undefined site

Mass spectrometry observed peptide