Prothrombin

Taxonomy

• Homo sapiens (Human)

Protein

• Prothrombin / Homo sapiens    P00734

Source

• Blood Plasma (UBERON_0001969)  
• Blood Serum (UBERON_0001977)  
• Mammary Gland (UBERON_0001911)  
• Urine (UBERON_0001088)  
• Cerebrospinal Fluid Secretion (GO_0033326)  

Reported structure

• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)Man(a1-3)[Gal(?1-?)GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Undefined core / HexNAc(??-?)HexNAc(??-?)[Hex(??-?)HexNAc(??-?)]Hex(??-?)[HexNAc(??-?)HexNAc(??-?)[HexNAc(??-?)HexNAc(??-?)]Hex(??-?)]Hex(??-?)HexNAc(??-?)HexNAc
• N-Linked / Complex / NeuAc(a2-6)Gal(b1-4)GlcNAc(b1-2)Man(a1-3)[Gal(b1-4)GlcNAc(b1-2)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / NeuAc(a2-6)Gal(b1-4)GlcNAc(b1-2)Man(a1-6)[Gal(b1-4)GlcNAc(b1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / NeuAc(?2-?)Gal(?1-?)GlcNAc(?1-?)Man(a1-3)[NeuAc(?2-?)Gal(?1-?)GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / NeuAc(a2-6)Gal(b1-4)GlcNAc(b1-2)Man(a1-3)[NeuAc(a2-6)Gal(b1-4)GlcNAc(b1-2)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc

Composition

• Hex:3 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2161.0134 )
• Hex:3 HexNAc:6 dHex:1 NeuAc:2 (avg mass : 2452.2713 )
• Hex:3 HexNAc:6 dHex:3 (avg mass : 2162.0415 )
• Hex:3 HexNAc:7 (avg mass : 1926.8075 )
• Hex:3 HexNAc:9 dHex:1 (avg mass : 2479.3405 )
• Hex:4 HexNAc:3 NeuAc:1 (avg mass : 1567.4278 )
• Hex:4 HexNAc:4 NeuAc:1 (avg mass : 1770.6228 )
• Hex:4 HexNAc:5 NeuAc:1 NeuGc:1
• Hex:4 HexNAc:9
• Hex:5 HexNAc:3 NeuAc:1 (avg mass : 1729.5702 )
• Hex:5 HexNAc:4 (avg mass : 1641.5073 )
• Hex:5 HexNAc:4 NeuAc:1 (avg mass : 1932.7652 )
• Hex:5 HexNAc:4 NeuAc:2 (avg mass : 2224.0231 )
• Hex:5 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 2078.9082 )
• Hex:5 HexNAc:4 dHex:1 NeuAc:2 (avg mass : 2370.1661 )
• Hex:5 HexNAc:4 dHex:2 NeuAc:1 (avg mass : 2225.0512 )
• Hex:5 HexNAc:4 dHex:2 NeuAc:2 (avg mass : 2516.3091 )
• Hex:5 HexNAc:4 dHex:3 NeuAc:1 (avg mass : 2371.1942 )
• Hex:5 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2282.1032 )
• Hex:5 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2485.2982 )
• Hex:6 HexNAc:4 dHex:1 (avg mass : 1949.7927 )
• Hex:6 HexNAc:5 NeuAc:2 (avg mass : 2589.3605 )
• Hex:6 HexNAc:5 NeuAc:3 (avg mass : 2880.6184 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:2 (avg mass : 2735.5035 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:3 (avg mass : 3026.7614 )
• Hex:6 HexNAc:5 dHex:2 (avg mass : 2299.1307 )
• Hex:6 HexNAc:6 (avg mass : 2210.0397 )
• Hex:6 HexNAc:6 NeuAc:2 (avg mass : 2792.5555 )
• Hex:6 HexNAc:6 dHex:1 (avg mass : 2356.1827 )
• Hex:6 HexNAc:6 dHex:1 NeuAc:2 (avg mass : 2938.6985 )
• Hex:6 HexNAc:6 dHex:2 NeuAc:2
• Hex:7 HexNAc:6 NeuAc:3 (avg mass : 3245.9558 )
• Hex:7 HexNAc:6 dHex:1 (avg mass : 2518.3251 )

Disease

• Cancer, breast (DOID:1612)
• Esophageal cancer (DOID:5041)
• Oral squamous cell carcinoma (DOID:0050866)
• Prostate cancer (DOID:10283)

Reference

• Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides (2018 - Yingwei Hu, Punit Shah, David J. Clark, Minghui Ao, Hui Zhang) / Status : Unreviewed
• Large-scale intact glycopeptide identification by Mascot database search (2018 - Ravi Chand Bollineni, Christian Jeffrey Koehler, Randi Elin Gislefoss, Jan Haug Anonsen, Bernd Thiede) / Status : Unreviewed
• Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein (2018 - Shisheng Sun, Yingwei Hu, Li Jia, Shadi Toghi Eshghi, Yang Liu, Punit Shah, Hui Zhang) / Status : Unreviewed
• Distinct urinary glycoprotein signatures in prostate cancer patients (2018 - Rebeca Kawahara, Fabio Ortega, Livia Rosa-Fernandes, Vanessa Guimarães, Daniel Quina, Willian Nahas, Veit Schwämmle, Miguel Srougi, Katia R.M. Leite, Morten Thaysen-Andersen, Martin R. Larsen and Giuseppe Palmisano) / Status : Unreviewed
• Tongue Cancer Patients Can be Distinguished from Healthy Controls by Specific N-Glycopeptides Found in Serum. (2018 - Mayank Saraswat, Antti Mäkitie, Tiialotta Tohmola, Amy Dickinson, Shruti Saraswat, Sakari Joenväärä, Suvi Renkonen) / Status : Reviewed
• Selective glycopeptide profiling by acetone enrichment and LC/MS (2014 - Takakura D, Harazono A, Hashii N, Kawasaki N) / Status : Reviewed
• Comparison of sialylated N-glycopeptide levels in serum of pancreatic cancer patients, acute pancreatitis patients, and healthy controls (2014 - Kontro H, Joenväärä S, Haglund C, Renkonen R) / Status : Reviewed
• Computational framework for identification of intact glycopeptides in complex samples (2014 - Mayampurath A, Yu CY, Song E, Balan J, Mechref Y, Tang H.) / Status : Reviewed
• Human urinary glycoproteomics; attachment site specific analysis of N- and O-linked glycosylations by CID and ECD (2012 - Halim A, Nilsson J, Rüetschi U, Hesse C, Larson G) / Status : Reviewed
• Enrichment of glycopeptides for glycan structure and attachment site identification (2009 - Nilsson J, Rüetschi U, Halim A, Hesse C, Carlsohn E, Brinkmalm G, Larson G.) / Status : Reviewed
• Studies on the structures of the carbohydrate moiety of human prothrombin. (1981 - Mizuochi T, Fujii J, Kisiel W, Kobata A) / Status : Reviewed

Reported glycosite

• Prothrombin / Homo sapiens
  •        Undefined site
  •        Asn-121
  •        Asn-143
  •        Asn-252
  •        Asn-416

Mass spectrometry observed peptide

• DKLAACLEGNCAEGLGTNYRGHVNITR (27aa)
  •    P00734    Asn-121
• GHVNITR (7aa)
  •    P00734    Asn-121
• GHVNITRSGIECQLWR (16aa)
  •    P00734    Asn-121
• YPHKPEINSTTHPGADIQENFCR (23aa)
  •    P00734    Asn-143
• YPHKPEINSTTHPGADLQENFCR (23aa)
  •    P00734    Asn-143
• HKPEINSTTHPGADLQENFCR (21aa)
  •    P00734    Asn-143
• SRYPHKPEINSTTHPGADLQENFCR (25aa)
  •    P00734    Asn-143
• YPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVR (42aa)
  •    P00734    Asn-143
• HQDFNSAVQLVENFCR (16aa)
  •    P00734    Asn-252
• NFTENDLLVR (10aa)
  •    P00734    Asn-416
• CLLYPPWDKNFTENDLLVR (19aa)
  •    P00734    Asn-416