Non-secretory ribonuclease

Taxonomy

• Homo sapiens (Human)
• Fusion - homo sapiens/sus scrofa

Protein

• Non-secretory ribonuclease / Homo sapiens    P10153

Source

• Kidney (UBERON_0002113)   COS-7 (CVCL_0224)  
• Kidney (UBERON_0002113)   HEK293-EBNA (CVCL_6974)  
• Kidney (UBERON_0002113)   LLC-PK1 (CVCL_0391)  
• Kidney (UBERON_0002113)  
• Liver (UBERON_0002107)  
• Ovary (UBERON_0000992)   CHO (CVCL_0213)  
• Spleen (UBERON_0002106)  
• Urine (UBERON_0001088)  
• HL-60 (CVCL_0002)   Leukocyte (CL_0000738)  
• NIH 3T3 (CVCL_0594)   Fibroblast (CL_0000057)  
• Erythrocyte (CL_0000232)  

Reported structure

• C-Linked / Undefined core / Man
• N-Linked / No-core / Fuc(a1-6)[GlcNAc(b1-4)]GlcNAc
• N-Linked / No-core / Man(a1-6)Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / No-core / GlcNAc(b1-2)Man(a1-6)Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc

Composition

• HexNAc:2 dHex:1 (avg mass : 570.5483 )
• Hex:1 (avg mass : 180.1577 )
• Hex:2 HexNAc:2 dHex:1 (avg mass : 894.8331 )
• Hex:2 HexNAc:3 dHex:1 (avg mass : 1098.0281 )

Disease

• Leukemia, Promyelocytic (DOID:0060318)

Reference

• Recognition signal for C-mannosylation of Trp-7 in RNase 2 consists of sequence Trp-x-x-Trp. (1998 - Krieg J, Hartmann S, Vicentini A, Glsner W, Hess D, Hofsteenge J) / Status : Reviewed
• C-Mannosylation of human RNase 2 is an intracellular process performed by a variety of cultured cells. (1997 - Krieg J, Glasner W, Vicentini A, Doucey M, Loffler A, Hess D, Hofsteenge J) / Status : Reviewed
• Spectroscopic and protein chemical analyses demonstrate the presence of C-mannosylated tryptophan in intact human RNase 2 and its isoforms. (1996 - Loffler A, Doucey M, Jansson A, Muller D, de Beer T, Hess D, Meldal M, Richter W, Vliegenthart J, Hofsteenge J) / Status : Reviewed
• The hexopyranosyl residue that is C-glycosidically linked to the side chain of tryptophan-7 in human RNase Us is alpha-mannopyranose. (1995 - de Beer T, Vliegenthart J, Loffler A, Hofsteenge J) / Status : Reviewed
• New type of linkage between a carbohydrate and a protein: C-glycosylation of a specific tryptophan residue in human RNase Us. (1994 - Hofsteenge J, Mller D, de Beer T, Lffler A, Richter W, Vliegenthart J) / Status : Reviewed
• Human non-secretory ribonucleases. II. Structural characterization of the N-glycans of the kidney, liver and spleen enzymes by NMR spectroscopy and electrospray mass spectrometry. (1993 - Lawrence C, Little P, Little B, Glushka J, van Halbeek H, Alhadeff J) / Status : Reviewed

Reported glycosite

• Non-secretory ribonuclease / Homo sapiens
  •        Undefined site
  •        Trp-34

Mass spectrometry observed peptide