GlyConnect

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Site	Core / Type	Cartoon	Composition	Biological associations
Ser-228	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide AVVPVSK (7aa) Q7Z589 Ser-228 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Ser-236	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide TITVPVSGSPK (11aa) Q7Z589 Ser-236 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Thr-271	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide PSTQTTNTTTQK (12aa) Q7Z589 Thr-271 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Thr-501	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide LVTTPTGTQATYTRPTVSPSIGR (23aa) Q7Z589 Thr-501 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Thr-506 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Thr-506	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide LVTTPTGTQATYTRPTVSPSIGR (23aa) Q7Z589 Thr-501 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Thr-506 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Thr-534	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide TTSGSIITVVPK (12aa) Q7Z589 Thr-535 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Thr-534 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Thr-535	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide TTSGSIITVVPK (12aa) Q7Z589 Thr-535 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Thr-534 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Ser-557	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide IISSNIVSGTTTK (13aa) Q7Z589 Ser-557 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Thr-1120	O-Linked / O-GlcNAc		HexNAc:1	taxonomy (1) protein (1) source (1) structure (1) composition (1) disease (0) reference (1) site (9) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source HeLa (CVCL_0030) Reported structure O-Linked / O-GlcNAc / GlcNAc(b Composition HexNAc:1 (avg mass : 221.2103 ) Disease Reference Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Ser-228 Ser-236 Thr-271 Thr-501 Thr-506 Thr-534 Thr-535 Ser-557 Thr-1120 Mass spectrometry observed peptide ITGGSSVPK (9aa) Q7Z589 Thr-1120 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Asn-1199	N-Linked / Undefined core		Hex:6 HexNAc:4 dHex:1 NeuAc:1	taxonomy (1) protein (1) source (1) structure (0) composition (1) disease (1) reference (1) site (1) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source Mammary Gland (UBERON_0001911) Reported structure Composition Hex:6 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 2241.0506 ) Disease Cancer, breast (DOID:1612) Reference Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides (2018 - Yingwei Hu, Punit Shah, David J. Clark, Minghui Ao, Hui Zhang) / Status : Unreviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Asn-1199 Mass spectrometry observed peptide NSTPQQQK (8aa) Q7Z589 Asn-1199 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
Asn-1199	N-Linked / Undefined core		Hex:6 HexNAc:6 dHex:1	taxonomy (1) protein (1) source (1) structure (0) composition (1) disease (1) reference (1) site (1) peptide (1) Taxonomy Homo sapiens (Human) Protein BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Q7Z589 Source Mammary Gland (UBERON_0001911) Reported structure Composition Hex:6 HexNAc:6 dHex:1 (avg mass : 2356.1827 ) Disease Cancer, breast (DOID:1612) Reference Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides (2018 - Yingwei Hu, Punit Shah, David J. Clark, Minghui Ao, Hui Zhang) / Status : Unreviewed Reported glycosite BRCA2-interacting transcriptional repressor EMSY / Homo sapiens Asn-1199 Mass spectrometry observed peptide NSTPQQQK (8aa) Q7Z589 Asn-1199 BRCA2-interacting transcriptional repressor EMSY / Homo sapiens

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