Complement c3

Taxonomy

• Homo sapiens (Human)

Protein

• Complement c3 / Homo sapiens    P01024

Source

• Blood Plasma (UBERON_0001969)  
• Blood Serum (UBERON_0001977)  
• Mammary Gland (UBERON_0001911)  
• Milk (UBERON_0001913)  

Reported structure

• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 2812
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 3485
• N-Linked / High-Mannose / Structure 314
• N-Linked / High-Mannose / Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)[Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-2)Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 3542
• N-Linked / High-Mannose / Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)[Man(a1-2)Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 2636
• N-Linked / Hybrid / Structure 9492
• N-Linked / Hybrid / Structure 9403
• N-Linked / Hybrid / Structure 3469

Composition

• Hex:1 HexNAc:1 NeuAc:3 (avg mass : 1257.1264 )
• Hex:3 HexNAc:3 (avg mass : 1114.0275 )
• Hex:3 HexNAc:6 (avg mass : 1723.6125 )
• Hex:3 HexNAc:7 (avg mass : 1926.8075 )
• Hex:4 HexNAc:2 (avg mass : 1072.9749 )
• Hex:4 HexNAc:3 (avg mass : 1276.1699 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:5 HexNAc:3 (avg mass : 1438.3123 )
• Hex:5 HexNAc:3 NeuAc:1 (avg mass : 1729.5702 )
• Hex:5 HexNAc:3 dHex:1 NeuAc:1 (avg mass : 1875.7132 )
• Hex:5 HexNAc:4 NeuAc:2 (avg mass : 2224.0231 )
• Hex:5 HexNAc:8 dHex:1 (avg mass : 2600.4303 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:6 HexNAc:3 (avg mass : 1600.4547 )
• Hex:6 HexNAc:3 NeuAc:1 (avg mass : 1891.7126 )
• Hex:6 HexNAc:5 dHex:3 NeuAc:1 (avg mass : 2736.5316 )
• Hex:6 HexNAc:6 (avg mass : 2210.0397 )
• Hex:6 HexNAc:6 dHex:1 (avg mass : 2356.1827 )
• Hex:7 HexNAc:2 (avg mass : 1559.4021 )
• Hex:7 HexNAc:6 dHex:1 (avg mass : 2518.3251 )
• Hex:7 HexNAc:6 dHex:1 NeuAc:4 (avg mass : 3683.3567 )
• Hex:7 HexNAc:6 dHex:2 (avg mass : 2664.4681 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )
• Hex:8 HexNAc:7 dHex:1 (avg mass : 2883.6625 )
• Hex:9 HexNAc:2 (avg mass : 1883.6869 )

Disease

• Cancer, breast (DOID:1612)
• Esophageal cancer (DOID:5041)
• Prostate cancer (DOID:10283)

Reference

• Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis (2021 - Rebeca Kawahara, Anastasia Chernykh, Kathirvel Alagesan, Marshall Bern, Weiqian Cao, Robert J. Chalkley, Kai Cheng, Matthew S. Choo, Nathan Edwards, Radoslav Goldman, Marcus Hoffmann, Yingwei Hu, Yifan Huang, Jin Young Kim, Doron Kletter, Benoit Liquet, Mingqi Liu, Yehia Mechref, Bo Meng, Sriram Neelamegham, Terry Nguyen-Khuong, Jonas Nilsson, Adam Pap, Gun Wook Park, Benjamin L. Parker, Cassandra L. Pegg, Josef M. Penninger, Toan K. Phung, Markus Pioch, Erdmann Rapp, Enes Sakalli, Miloslav Sanda, Benjamin L. Schulz, Nichollas E. Scott, Georgy Sofronov, Johannes Stadlmann, Sergey Y. Vakhrushev, Christina M. Woo, Hung-Yi Wu, Pengyuan Yang, Wantao Ying, Hui Zhang, Yong Zhang, Jingfu Zhao, Joseph Zaia, Stuart M. Haslam, Giuseppe Palmisano, Jong Shin Yoo, Göran Larson, Kai-Hooi Khoo, Katalin F. Medzihradszky, Daniel Kolarich, Nicolle H. Packer, Morten Thaysen-Andersen) / Status : Reviewed
• Quantitative Longitudinal Inventory of the N-Glycoproteome of Human Milk from a Single Donor Reveals the Highly Variable Repertoire and Dynamic Site-Specific Changes (2020 - Jing Zhu, Yu-Hsien Lin, Kelly A Dingess, Marko Mank, Bernd Stahl, Albert J R Heck) / Status : Reviewed
• Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein (2018 - Shisheng Sun, Yingwei Hu, Li Jia, Shadi Toghi Eshghi, Yang Liu, Punit Shah, Hui Zhang) / Status : Unreviewed
• Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides (2018 - Yingwei Hu, Punit Shah, David J. Clark, Minghui Ao, Hui Zhang) / Status : Unreviewed
• Large-scale intact glycopeptide identification by Mascot database search (2018 - Ravi Chand Bollineni, Christian Jeffrey Koehler, Randi Elin Gislefoss, Jan Haug Anonsen, Bernd Thiede) / Status : Unreviewed
• Selective glycopeptide profiling by acetone enrichment and LC/MS (2014 - Takakura D, Harazono A, Hashii N, Kawasaki N) / Status : Reviewed
• Computational framework for identification of intact glycopeptides in complex samples (2014 - Mayampurath A, Yu CY, Song E, Balan J, Mechref Y, Tang H.) / Status : Reviewed
• Simultaneous glycosylation analysis of human serum glycoproteins by high-performance liquid chromatography/tandem mass spectrometry (2008 - Harazono A, Kawasaki N, Itoh S, Hashii N, Matsuishi-Nakajima Y, Kawanishi T, Yamaguchi T) / Status : Reviewed
• Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins. (1987 - Hase S, Natsuka S, Oku H, Ikenaka T) / Status : Reviewed

Reported glycosite

• Complement c3 / Homo sapiens
  •        Undefined site
  •        Asn-85
  •        Thr-87
  •        Asn-939
  •        Asn-1617

Mass spectrometry observed peptide

• TVLTPATNHMGNVTFTIPANREFK (24aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• TVITPATNHMGNVTFTIPANR (21aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• TNHMGNVTFTIPANR (15aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• MGNVTFTIPANR (12aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• KTVLTPATNHMGNVTFTIPANRE (23aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• TVLTPATNHMGNVTFTIPANREF (23aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• KTVLTPATNHMGNVTFTIPANREFKS (26aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• NHMGNVTFTIPANR (14aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• TVLTPATNHMGNVTF (15aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• HMGNVTFTIPANR (13aa)
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• TVLTPATNHMGNVTFTIPANR (21aa)
  •    P01024    Thr-87     Complement c3 / Homo sapiens
  •    P01024    Asn-85     Complement c3 / Homo sapiens
• VVPEGIRMNK (10aa)
  •    P01024    Asn-939     Complement c3 / Homo sapiens
• GIRMNKTVAVRTLDPE (16aa)
  •    P01024    Asn-939     Complement c3 / Homo sapiens
• HYLMWGLSSDFWGEKPNLSYIIGK (24aa)
  •    P01024    Asn-1617     Complement c3 / Homo sapiens