HAIHVSGTNGTK (12aa)

Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• BTI-Tn-5B1-4 (CVCL_C190)  
• HEK293-F (CVCL_6642)  
• Schneider 2 (CVCL_Z232)  

Reported structure

• O-Linked / Core 0 / GalNAc
• O-Linked / Core 1 / Gal(b1-3)GalNAc

Composition

• HexNAc:1 (avg mass : 221.2103 )
• Hex:1 HexNAc:1 (avg mass : 383.3527 )
• Hex:2 HexNAc:2 (avg mass : 748.6901 )
• Hex:2 HexNAc:2 dHex:1 (avg mass : 894.8331 )
• Hex:2 HexNAc:3 dHex:1 (avg mass : 1098.0281 )
• Hex:3 HexNAc:2 (avg mass : 910.8325 )
• Hex:3 HexNAc:2 dHex:1 (avg mass : 1056.9755 )
• Hex:3 HexNAc:3 dHex:1 (avg mass : 1260.1705 )
• Hex:3 HexNAc:3 dHex:2 (avg mass : 1406.3135 )
• Hex:3 HexNAc:4 dHex:2 (avg mass : 1609.5085 )
• Hex:4 HexNAc:3 (avg mass : 1276.1699 )
• Hex:4 HexNAc:3 dHex:2 (avg mass : 1568.4559 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:5 HexNAc:2 dHex:2 (avg mass : 1527.4033 )
• Hex:5 HexNAc:3 (avg mass : 1438.3123 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:6 HexNAc:3 (avg mass : 1600.4547 )
• Hex:7 HexNAc:2 (avg mass : 1559.4021 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )
• Hex:9 HexNAc:2 (avg mass : 1883.6869 )
• Hex:10 HexNAc:2 (avg mass : 2045.8293 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed
• Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics (2020 - Dapeng Zhou, Xiaoxu Tian, Ruibing Qi, Chao Peng, Wen Zhang) / Status : Reviewed

Reported glycosite

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-74
• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-73
  •        Thr-76
• Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Ser-71
  •        Ser-71
  •        Thr-73
  •        Thr-76

Mass spectrometry observed peptide

• HAIHVSGTNGTK (12aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-74     Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)