NHTSPDVDLGDISGINASVVNIQK (24aa)

Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• BTI-Tn-5B1-4 (CVCL_C190)  

Reported structure

• N-Linked / Complex / Structure 10054
• N-Linked / High-Mannose / Structure 10055
• N-Linked / High-Mannose / Structure 10030
• N-Linked / High-Mannose / Structure 10028
• N-Linked / Hybrid / Structure 10003
• N-Linked / Pauci-Mannose / Structure 10056
• N-Linked / Pauci-Mannose / Structure 10069
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Pauci-Mannose / Structure 9617
• N-Linked / Pauci-Mannose / Structure 10068
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-3)][Fuc(a1-6)]GlcNAc
• N-Linked / Pauci-Mannose / Structure 10067
• N-Linked / Pauci-Mannose / Structure 10066
• N-Linked / Truncated / Structure 9993
• N-Linked / Truncated / Structure 9992

Composition

• HexNAc:1 dHex:1 (avg mass : 367.3533 )
• HexNAc:1 dHex:2 (avg mass : 513.4963 )
• Hex:1 HexNAc:2 dHex:2 (avg mass : 878.8337 )
• Hex:2 HexNAc:2 dHex:1 (avg mass : 894.8331 )
• Hex:2 HexNAc:2 dHex:2 (avg mass : 1040.9761 )
• Hex:3 HexNAc:2 (avg mass : 910.8325 )
• Hex:3 HexNAc:2 dHex:1 (avg mass : 1056.9755 )
• Hex:3 HexNAc:2 dHex:2 (avg mass : 1203.1185 )
• Hex:3 HexNAc:3 (avg mass : 1114.0275 )
• Hex:3 HexNAc:3 dHex:1 (avg mass : 1260.1705 )
• Hex:3 HexNAc:3 dHex:2 (avg mass : 1406.3135 )
• Hex:4 HexNAc:2 dHex:2 (avg mass : 1365.2609 )
• Hex:4 HexNAc:3 (avg mass : 1276.1699 )
• Hex:4 HexNAc:3 dHex:1 (avg mass : 1422.3129 )
• Hex:4 HexNAc:3 dHex:2 (avg mass : 1568.4559 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:5 HexNAc:2 dHex:1 (avg mass : 1381.2603 )
• Hex:5 HexNAc:2 dHex:2 (avg mass : 1527.4033 )
• Hex:5 HexNAc:3 dHex:2 (avg mass : 1730.5983 )
• Hex:5 HexNAc:4 dHex:3 (avg mass : 2079.9363 )
• Hex:5 HexNAc:4 dHex:4 (avg mass : 2226.0793 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:6 HexNAc:4 dHex:3 (avg mass : 2242.0787 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Reviewed
• Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics (2020 - Dapeng Zhou, Xiaoxu Tian, Ruibing Qi, Chao Peng, Wen Zhang) / Status : Reviewed

Reported glycosite

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-1158
  •        Asn-1159
  •        Asn-1173
  •        Asn-1174

Mass spectrometry observed peptide

• NHTSPDVDLGDISGINASVVNIQK (24aa)
  •    P0DTC2    Asn-1174     Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-1158     Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-1159     Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-1173     Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)