VFNATR (6aa)

Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• BTI-Tn-5B1-4 (CVCL_C190)  
• HEK293 (CVCL_0045)  

Reported structure

• N-Linked / Complex / GlcNAc(?1-?)Man(a1-3)[GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-?)]GlcNAc
• N-Linked / Complex / Structure 2719
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)Man(a1-3)[Gal(?1-?)GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / Structure 10064
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)Man(a1-3)[Gal(?1-?)GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Complex / Structure 10049
• N-Linked / Complex / Structure 9713
• N-Linked / Complex / Structure 10022
• N-Linked / Complex / Structure 3507
• N-Linked / Complex / Structure 3446
• N-Linked / Complex / Structure 1523
• N-Linked / Complex / Structure 10020
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)[Gal(?1-?)GlcNAc(?1-?)]Man(a1-?)[Gal(?1-?)GlcNAc(?1-?)Man(a1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Complex / Structure 9663
• N-Linked / Complex / Structure 1149
• N-Linked / Complex / Structure 1519
• N-Linked / Complex / Structure 10061
• N-Linked / Complex / Structure 10057
• N-Linked / Complex / Structure 10040
• N-Linked / High-Mannose / Structure 2812
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 314
• N-Linked / High-Mannose / Structure 2636
• N-Linked / High-Mannose / Structure 10029
• N-Linked / Hybrid / Structure 10026
• N-Linked / Hybrid / Structure 1713
• N-Linked / Hybrid / Structure 9730
• N-Linked / Hybrid / Structure 10023
• N-Linked / Hybrid / Structure 10010
• N-Linked / Hybrid / Structure 10008
• N-Linked / Pauci-Mannose / Structure 9617
• N-Linked / Pauci-Mannose / Structure 10068
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ GlcNAc"
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-3)][Fuc(a1-6)]GlcNAc
• N-Linked / Pauci-Mannose / Structure 10067

Composition

• Hex:2 HexNAc:2 dHex:2 (avg mass : 1040.9761 )
• Hex:3 HexNAc:2 dHex:1 (avg mass : 1056.9755 )
• Hex:3 HexNAc:2 dHex:2 (avg mass : 1203.1185 )
• Hex:3 HexNAc:3 (avg mass : 1114.0275 )
• Hex:3 HexNAc:3 dHex:1 (avg mass : 1260.1705 )
• Hex:3 HexNAc:4 dHex:1 (avg mass : 1463.3655 )
• Hex:3 HexNAc:5 dHex:1 (avg mass : 1666.5605 )
• Hex:3 HexNAc:6 dHex:1 (avg mass : 1869.7555 )
• Hex:4 HexNAc:3 dHex:1 (avg mass : 1422.3129 )
• Hex:4 HexNAc:4 dHex:1 (avg mass : 1625.5079 )
• Hex:4 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 1916.7658 )
• Hex:4 HexNAc:5 dHex:1 (avg mass : 1828.7029 )
• Hex:4 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2119.9608 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:5 HexNAc:3 (avg mass : 1438.3123 )
• Hex:5 HexNAc:3 dHex:1 (avg mass : 1584.4553 )
• Hex:5 HexNAc:4 (avg mass : 1641.5073 )
• Hex:5 HexNAc:4 NeuAc:1 (avg mass : 1932.7652 )
• Hex:5 HexNAc:4 dHex:1 (avg mass : 1787.6503 )
• Hex:5 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 2078.9082 )
• Hex:5 HexNAc:4 dHex:1 NeuAc:2 (avg mass : 2370.1661 )
• Hex:5 HexNAc:5 dHex:1 (avg mass : 1990.8453 )
• Hex:5 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2282.1032 )
• Hex:5 HexNAc:6 dHex:1 (avg mass : 2194.0403 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:6 HexNAc:3 (avg mass : 1600.4547 )
• Hex:6 HexNAc:3 dHex:1 (avg mass : 1746.5977 )
• Hex:6 HexNAc:4 dHex:1 (avg mass : 1949.7927 )
• Hex:6 HexNAc:5 dHex:1 (avg mass : 2152.9877 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2444.2456 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:2 (avg mass : 2735.5035 )
• Hex:6 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2647.4406 )
• Hex:7 HexNAc:2 (avg mass : 1559.4021 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )
• Hex:9 HexNAc:2 (avg mass : 1883.6869 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Reviewed

Reported glycosite

• Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-343
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-343

Mass spectrometry observed peptide

• VFNATR (6aa)
  •    P0DTC2    Asn-343     Recombinant Spike glycoprotein (BTI-Tn-5B1-4) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Asn-343     Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)