Hex:9 HexNAc:6 dHex:1 NeuAc:2

Taxonomy

• Homo sapiens (Human)
• Bos taurus (Bovine)
• Mus musculus (House mouse)
• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Alpha-2-HS-glycoprotein / Homo sapiens    P02765
• Attractin / Homo sapiens    O75882
• Kininogen-1 / Homo sapiens    P01042
• Lysosome membrane protein 2 / Homo sapiens    Q14108
• Lysosome-associated membrane glycoprotein 2 / Homo sapiens    P13473
• N-acetylglucosamine-6-sulfatase / Homo sapiens    P15586
• Recombinant mature angiotensin-converting enzyme 2 - DRAFT DATA / Homo sapiens    Q9BYF1
• Sodium/potassium-transporting ATPase subunit beta-1 / Homo sapiens    P05026
• Thioredoxin domain-containing protein 15 / Homo sapiens    Q96J42
• Uromodulin / Homo sapiens    P07911
• Uncharacterized protein from Plasma Membrane / Bos taurus
• Lysosome-associated membrane glycoprotein 1 / Mus musculus    P11438
• Recombinant Spike glycoprotein (HEK293) - DRAFT DATA / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• Brain (UBERON_0000955)  
• Mammary Gland (UBERON_0001911)  
• Urine (UBERON_0001088)  
• HEK293 (CVCL_0045)  
• Plasma Membrane (GO_0005886)  

Reported structure

• N-Linked / Complex / NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-2)[NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-6)]Man(a1-3)[Gal(b1-3)Gal(b1-4)GlcNAc(b1-2)[Gal(b1-3)Gal(b1-4)GlcNAc(b1-4)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc

Composition

• Hex:9 HexNAc:6 dHex:1 NeuAc:2 (avg mass : 3425.1257 )

Disease

• Cancer, breast (DOID:1612)
• COVID-19 (DOID:0080600)
• Prostate cancer (DOID:10283)

Reference

• Comprehensive characterization of N- and O- glycosylation of SARS-CoV-2 human receptor angiotensin converting enzyme 2. (2020 - Asif Shajahan, Stephanie Archer-Hartmann, Nitin T. Supekar, Anne S. Gleinich, Christian Heiss, and Parastoo Azadi) / Status : Unreviewed
• Deducing the N- and O- glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 (2020 - Asif Shajahan, Nitin T. Supekar, Anne S. Gleinich, Parastoo Azadi) / Status : Unreviewed
• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Unreviewed
• Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis (2019 - Nicholas M. Riley, Alexander S. Hebert, Michael S. Westphall & Joshua J. Coon ) / Status : Unreviewed
• Distinct urinary glycoprotein signatures in prostate cancer patients (2018 - Rebeca Kawahara, Fabio Ortega, Livia Rosa-Fernandes, Vanessa Guimarães, Daniel Quina, Willian Nahas, Veit Schwämmle, Miguel Srougi, Katia R.M. Leite, Morten Thaysen-Andersen, Martin R. Larsen and Giuseppe Palmisano) / Status : Unreviewed
• Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides (2018 - Yingwei Hu, Punit Shah, David J. Clark, Minghui Ao, Hui Zhang) / Status : Unreviewed
• The asparagine-linked sugar chains of the glycoproteins in calf thymocyte plasma membrane. Structural studies of acidic oligosaccharides. (1980 - Yoshima H, Takasaki S, Kobata A) / Status : Reviewed

Reported glycosite

• Alpha-2-HS-glycoprotein / Homo sapiens
  •        Asn-176
• Attractin / Homo sapiens
  •        Asn-914
• Kininogen-1 / Homo sapiens
  •        Asn-294
• Lysosome membrane protein 2 / Homo sapiens
  •        Asn-206
• Lysosome-associated membrane glycoprotein 2 / Homo sapiens
  •        Asn-32
• N-acetylglucosamine-6-sulfatase / Homo sapiens
  •        Asn-111
• Recombinant mature angiotensin-converting enzyme 2 - DRAFT DATA / Homo sapiens
  •        Asn-322
• Sodium/potassium-transporting ATPase subunit beta-1 / Homo sapiens
  •        Asn-265
• Thioredoxin domain-containing protein 15 / Homo sapiens
  •        Asn-181
• Uromodulin / Homo sapiens
  •        Asn-513
• Uncharacterized protein from Plasma Membrane / Bos taurus
  •        Undefined site
• Lysosome-associated membrane glycoprotein 1 / Mus musculus
  •        Asn-78
• Recombinant Spike glycoprotein (HEK293) - DRAFT DATA / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-122
  •        Asn-149
  •        Asn-165
  •        Asn-234
  •        Asn-282
  •        Asn-331
  •        Asn-343
  •        Asn-657
  •        Asn-709
  •        Asn-1159
  •        Asn-1174
  •        Asn-1194

Mass spectrometry observed peptide

• LELNLTDSENATCLYAK (17aa)
  •    P13473    Asn-32
• ENVSDPSLTITFGR (14aa)
  •    P11438    Asn-78
• YPHNHHVVNNTIEGNCSSK (19aa)
  •    P15586    Asn-111
• TQSLLIVNNATNVVIK (16aa)
  •    P0DTC2    Asn-122
• NNKSWMESEFRVYSSANNC (19aa)
  •    P0DTC2    Asn-165
• NN (2aa)
  •    P0DTC2    Asn-149
• VYSSANNC (8aa)
  •    P0DTC2    Asn-165
• VYSSANNCTFE (11aa)
  •    P0DTC2    Asn-165
• AALAAFNAQNNGSNFQLEEISR (22aa)
  •    P02765    Asn-176
• NITGLENFTLK (11aa)
  •    Q96J42    Asn-181
• NGTNDGDYVFITGEDSYINFTK (22aa)
  •    Q14108    Asn-206
• VDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPR (47aa)
  •    P0DTC2    Asn-234
• YIQPIIAVQFTNITMDTEIR (20aa)
  •    P05026    Asn-265
• NGTITDAVDCALDPLSE (17aa)
  •    P0DTC2    Asn-282
• LNAENNATFYFK (12aa)
  •    P01042    Asn-294
• VSVGLPNM (8aa)
  •    Q9BYF1    Asn-322
• FPNITNLCPFGE (12aa)
  •    P0DTC2    Asn-331
• NITNLCPFGEVFNATR (16aa)
  •    P0DTC2    Asn-343
• FALLMTNCYATPSSNATDPLK (21aa)
  •    P07911    Asn-513
• AGCLIGAEHVNNSYE (15aa)
  •    P0DTC2    Asn-657
• AGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR (36aa)
  •    P0DTC2    Asn-657
• SIIAYTMSLGAENSVAYSNNSIAIPTNFT (29aa)
  •    P0DTC2    Asn-709
• AATCINPINGSVCERPANHSAK (22aa)
  •    O75882    Asn-914
• YFKNHTSPDVDLGDISGINASVVNIQK (27aa)
  •    P0DTC2    Asn-1159
• NHTSPDVDLGDISGIN (16aa)
  •    P0DTC2    Asn-1174
• NHTSPDVDLGDISGIN (16aa)
  •    P0DTC2    Asn-1159
• LNEVAKNLNE (10aa)
  •    P0DTC2    Asn-1194