Hex:4 HexNAc:5 NeuAc:2

Taxonomy

• Homo sapiens (Human)
• Mus musculus (House mouse)
• Columba livia (Domestic pigeon)
• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Alpha-S1-casein / Homo sapiens    P47710
• Biglycan / Homo sapiens    P21810
• Chordin-like protein 2 / Homo sapiens    Q6WN34
• Clusterin / Homo sapiens    P10909
• Complement c4-a / Homo sapiens    P0C0L4
• Complement C4-B / Homo sapiens    P0C0L5
• Complement factor b / Homo sapiens    P00751
• Fibronectin / Homo sapiens    P02751
• Glycoprotein hormones alpha chain - choriogonadotropin / Homo sapiens    P01215
• Golgi apparatus protein 1 / Homo sapiens    Q92896
• Haptoglobin / Homo sapiens    P00738
• Lactotransferrin / Homo sapiens    P02788
• Leucine-rich repeat transmembrane protein FLRT3 / Homo sapiens    Q9NZU0
• Lutropin beta chain / Homo sapiens    P01229
• Polymeric immunoglobulin receptor / Homo sapiens    P01833
• Prosaposin / Homo sapiens    P07602
• Prostaglandin-h2 d-isomerase / Homo sapiens    P41222
• Prostate-specific antigen (psa-1) / Homo sapiens    P07288
• Prostate-specific antigen - high Pi isoform (psah) / Homo sapiens    P07288
• Tenascin / Homo sapiens    P24821
• Thrombospondin-1 / Homo sapiens    P07996
• Uromodulin / Homo sapiens    P07911
• Zinc-alpha-2-glycoprotein / Homo sapiens    P25311
• Myelin-oligodendrocyte glycoprotein / Mus musculus    Q61885
• Thy-1 membrane glycoprotein / Mus musculus    P01831
• Immunoglobulin gamma / Columba livia    
• Recombinant Spike glycoprotein (FreeStyle 293-F) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• Brain (UBERON_0000955)  
• Mammary Gland (UBERON_0001911)  
• Milk (UBERON_0001913)  
• Pituitary Gland (UBERON_0000007)  
• Seminal Fluid (UBERON_0006530)  
• Umbilical Vein (UBERON_0002066)   Endothelial Cell of Umbilical Vein (CL_0002618)  
• Urine (UBERON_0001088)  
• FreeStyle 293-F (CVCL_D603)  
• HEK293 (CVCL_0045)  

Reported structure

• N-Linked / Complex / NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-2)Man(a1-6)[NeuAc(a2-6)GalNAc(b1-4)GlcNAc(b1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / NeuAc(a2-6)Gal(b1-4)GlcNAc(b1-2)Man(a1-6)[NeuAc(a2-6)HexNAc(b1-?)GlcNAc(b1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / Structure 9368
• N-Linked / Complex / Structure 9648
• N-Linked / Complex / Structure 9824
• N-Linked / Complex / Structure 10019
• N-Linked / Complex / Structure 10702
• N-Linked / Complex / Structure 10732
• N-Linked / Complex / Structure 10995

Composition

• Hex:4 HexNAc:5 NeuAc:2 (avg mass : 2265.0757 )

Disease

• Cancer, breast (DOID:1612)
• COVID-19 (DOID:0080600)
• Prostate cancer (DOID:10283)
• Prostate Disease (DOID:47)

Reference

• Profiling the proteoforms of urinary prostate-specific antigen by capillary electrophoresis – mass spectrometry (2021 - Alan B. Moran, Elena Domínguez-Vega, Jan Nouta, Tamas Pongracz, Theo M. de Reijke, Manfred Wuhrer, Guinevere S.M. Lageveen-Kammeijer) / Status : Reviewed
• High-throughput glycopeptide profiling of prostate-specific antigen from seminal plasma by MALDI-MS. (2021 - Wei Wang, Anna Kałuża, Jan Nouta, Simone Nicolardi, Mirosława Ferens-Sieczkowska, Manfred Wuhrer, Guinevere S M Lageveen-Kammeijer, Noortje de Haan) / Status : Reviewed
• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Reviewed
• Site-specific glycan analysis of the SARS-CoV-2 spike (2020 - Yasunori Watanabe, Joel D. Allen, Daniel Wrapp, Jason S. McLellan, Max Crispin) / Status : Unreviewed
• Quantitative Longitudinal Inventory of the N-Glycoproteome of Human Milk from a Single Donor Reveals the Highly Variable Repertoire and Dynamic Site-Specific Changes (2020 - Jing Zhu, Yu-Hsien Lin, Kelly A Dingess, Marko Mank, Bernd Stahl, Albert J R Heck) / Status : Reviewed
• Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis (2019 - Nicholas M. Riley, Alexander S. Hebert, Michael S. Westphall & Joshua J. Coon ) / Status : Unreviewed
• Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides (2018 - Yingwei Hu, Punit Shah, David J. Clark, Minghui Ao, Hui Zhang) / Status : Unreviewed
• Distinct urinary glycoprotein signatures in prostate cancer patients (2018 - Rebeca Kawahara, Fabio Ortega, Livia Rosa-Fernandes, Vanessa Guimarães, Daniel Quina, Willian Nahas, Veit Schwämmle, Miguel Srougi, Katia R.M. Leite, Morten Thaysen-Andersen, Martin R. Larsen and Giuseppe Palmisano) / Status : Unreviewed
• Glycoproteomics: Identifying the Glycosylation of Prostate Specific Antigen at Normal and High Isoelectric Points by LC-MS/MS (2014 - Song E, Mayampurath A, Yu CY, Tang H, Mechref Y) / Status : Reviewed
• Glycoproteomic analysis of the secretome of human endothelial cells (2013 - Yin X, Bern M, Xing Q, Ho J, Viner R, Mayr M.) / Status : Reviewed
• N-glycan structures of pigeon IgG: a major serum glycoprotein containing Galalpha1-4 Gal termini.. (2003 - Suzuki N, Khoo KH, Chen CM, Chen HC, Lee YC) / Status : Reviewed
• NMR investigations of the N-linked oligosaccharides at individual glycosylation sites of human lutropin. (1991 - Weisshaar G, Hiyama J, Renwick A, Nimtz M) / Status : Reviewed

Reported glycosite

• Alpha-S1-casein / Homo sapiens
  •        Asn-69
• Biglycan / Homo sapiens
  •        Asn-311
• Chordin-like protein 2 / Homo sapiens
  •        Asn-114
• Clusterin / Homo sapiens
  •        Asn-86
  •        Asn-374
• Complement c4-a / Homo sapiens
  •        Asn-1391
• Complement C4-B / Homo sapiens
  •        Asn-1391
• Complement factor b / Homo sapiens
  •        Asn-285
• Fibronectin / Homo sapiens
  •        Asn-528
• Glycoprotein hormones alpha chain - choriogonadotropin / Homo sapiens
  •        Asn-76
  •        Asn-102
• Golgi apparatus protein 1 / Homo sapiens
  •        Asn-165
• Haptoglobin / Homo sapiens
  •        Asn-184
  •        Asn-211
• Lactotransferrin / Homo sapiens
  •        Asn-497
• Leucine-rich repeat transmembrane protein FLRT3 / Homo sapiens
  •        Asn-226
• Lutropin beta chain / Homo sapiens
  •        Asn-50
• Polymeric immunoglobulin receptor / Homo sapiens
  •        Asn-90
• Prosaposin / Homo sapiens
  •        Asn-426
• Prostaglandin-h2 d-isomerase / Homo sapiens
  •        Asn-78
• Prostate-specific antigen (psa-1) / Homo sapiens
  •        Asn-69
• Prostate-specific antigen - high Pi isoform (psah) / Homo sapiens
  •        Asn-69
• Tenascin / Homo sapiens
  •        Asn-38
• Thrombospondin-1 / Homo sapiens
  •        Asn-1067
• Uromodulin / Homo sapiens
  •        Asn-513
• Zinc-alpha-2-glycoprotein / Homo sapiens
  •        Asn-128
• Myelin-oligodendrocyte glycoprotein / Mus musculus
  •        Asn-59
• Thy-1 membrane glycoprotein / Mus musculus
  •        Asn-42
• Immunoglobulin gamma / Columba livia
  •        Undefined site
• Recombinant Spike glycoprotein (FreeStyle 293-F) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-74
  •        Asn-331
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-282

Mass spectrometry observed peptide

• RQSGVNATLPEENQPVVFNHVYNIKL (26aa)
  •    P24821    Asn-38     Tenascin / Homo sapiens
• HENNTKDNSIQHEFSLTR (18aa)
  •    P01831    Asn-42     Thy-1 membrane glycoprotein / Mus musculus
• ISPGKNATGMEVGWYR (16aa)
  •    Q61885    Asn-59     Myelin-oligodendrocyte glycoprotein / Mus musculus
• AVCGGVLVHPQWVLTAAHCIRNK (23aa)
  •    P07288    Asn-69     Prostate-specific antigen - high Pi isoform (psah) / Homo sapiens
• NK (2aa)
  •    P07288    Asn-69     Prostate-specific antigen (psa-1) / Homo sapiens
• NKSVLLGR (8aa)
  •    P07288    Asn-69     Prostate-specific antigen - high Pi isoform (psah) / Homo sapiens
  •    P07288    Asn-69     Prostate-specific antigen (psa-1) / Homo sapiens
• KDTRNESTQNCVVAEPEKM (19aa)
  •    P47710    Asn-69     Alpha-S1-casein / Homo sapiens
• FHAIHVSGTNGTKRF (15aa)
  •    P0DTC2    Asn-74     Recombinant Spike glycoprotein (FreeStyle 293-F) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• SVVAPATDGGLNLTSTFLR (19aa)
  •    P41222    Asn-78     Prostaglandin-h2 d-isomerase / Homo sapiens
• KEDALNETR (9aa)
  •    P10909    Asn-86     Clusterin / Homo sapiens
• RANLTNFPENGTFVVNIAQLSQDDSGRY (28aa)
  •    P01833    Asn-90     Polymeric immunoglobulin receptor / Homo sapiens
• KSCQHNGTMYQHGEIFSAHELFPSRL (26aa)
  •    Q6WN34    Asn-114     Chordin-like protein 2 / Homo sapiens
• FGCEIENNR (9aa)
  •    P25311    Asn-128     Zinc-alpha-2-glycoprotein / Homo sapiens
• LNLTTDPK (8aa)
  •    Q92896    Asn-165     Golgi apparatus protein 1 / Homo sapiens
• KMVSHHNLTTGATLINEQWLLTTAKN (26aa)
  •    P00738    Asn-184     Haptoglobin / Homo sapiens
• KNLFLNHSENATAKD (15aa)
  •    P00738    Asn-211     Haptoglobin / Homo sapiens
• VFFNLVNLTELSLVR (15aa)
  •    Q9NZU0    Asn-226     Leucine-rich repeat transmembrane protein FLRT3 / Homo sapiens
• NGTITDAVDCALDPLSE (17aa)
  •    P0DTC2    Asn-282     Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• IYLVLDGSDSIGASNFTGAK (20aa)
  •    P00751    Asn-285     Complement factor b / Homo sapiens
• IIQVVYIHSNNITK (14aa)
  •    P21810    Asn-311     Biglycan / Homo sapiens
• RFPNIT (6aa)
  •    P0DTC2    Asn-331     Recombinant Spike glycoprotein (FreeStyle 293-F) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• IANITQGEDQYYIR (14aa)
  •    P10909    Asn-374     Clusterin / Homo sapiens
• TAGWNIPMGIIFNQTGSCK (19aa)
  •    P02788    Asn-497     Lactotransferrin / Homo sapiens
• FALLMTNCYATPSSNATDPLK (21aa)
  •    P07911    Asn-513     Uromodulin / Homo sapiens
• DQCIVDDITYNVNDTFHK (18aa)
  •    P02751    Asn-528     Fibronectin / Homo sapiens
• VVNSTTGPGEHIR (13aa)
  •    P07996    Asn-1067     Thrombospondin-1 / Homo sapiens
• NTTCQDLQIEVTVK (14aa)
  •    P0C0L4    Asn-1391     Complement c4-a / Homo sapiens
  •    P0C0L5    Asn-1391     Complement C4-B / Homo sapiens