Hex:7 HexNAc:7 dHex:1 NeuAc:3

Taxonomy

• Homo sapiens (Human)
• Rattus norvegicus (Norway rat)
• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Uromodulin / Homo sapiens    P07911
• Neuroligin 1 / Rattus norvegicus    Q62765
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• Kidney (UBERON_0002113)   HEK293-EBNA (CVCL_6974)  
• Urine (UBERON_0001088)  
• HEK293 (CVCL_0045)  

Reported structure

• N-Linked / Undefined core / Hex(?1-?)HexNAc(?1-?)[NeuAc(?2-?)Hex(?1-?)HexNAc(?1-?)]Man(a1-?)[NeuAc(?2-?)Hex(?1-?)HexNAc(?1-?)[NeuAc(?2-?)Hex(?1-?)HexNAc(?1-?)]Man(a1-?)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc+"+ HexNAc"

Composition

• Hex:7 HexNAc:7 dHex:1 NeuAc:3 (avg mass : 3595.2938 )

Disease

• COVID-19 (DOID:0080600)
• Prostate cancer (DOID:10283)

Reference

• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Unreviewed
• Distinct urinary glycoprotein signatures in prostate cancer patients (2018 - Rebeca Kawahara, Fabio Ortega, Livia Rosa-Fernandes, Vanessa Guimarães, Daniel Quina, Willian Nahas, Veit Schwämmle, Miguel Srougi, Katia R.M. Leite, Morten Thaysen-Andersen, Martin R. Larsen and Giuseppe Palmisano) / Status : Unreviewed
• Structural characterization of recombinant soluble rat neuroligin 1: mapping of secondary structure and glycosylation by mass spectrometry. (2004 - Hoffman RC, Jennings LL, Tsigelny I, Comoletti D, Flynn RE, Sudhof TC, Taylor P) / Status : Reviewed

Reported glycosite

• Uromodulin / Homo sapiens
  •        Asn-322
• Neuroligin 1 / Rattus norvegicus
  •        Undefined site
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-122
  •        Asn-165
  •        Asn-282
  •        Asn-657

Mass spectrometry observed peptide

• TQSLLIVNNATNVVIK (16aa)
  •    P0DTC2    Asn-122
• VYSSANNCTFE (11aa)
  •    P0DTC2    Asn-165
• NGTITDAVDCALDPLSE (17aa)
  •    P0DTC2    Asn-282
• QDFNITDISLLEHR (14aa)
  •    P07911    Asn-322
• AGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR (36aa)
  •    P0DTC2    Asn-657
• AGCLIGAEHVNNSYE (15aa)
  •    P0DTC2    Asn-657