Hex:7 HexNAc:7 dHex:1 NeuAc:2

Taxonomy

• Homo sapiens (Human)
• Rattus norvegicus (Norway rat)
• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Alpha-1-acid glycoprotein 1 / Homo sapiens    P02763
• Alpha-1-acid glycoprotein 2 / Homo sapiens    P19652
• Lumican / Homo sapiens    P51884
• Neuroligin 1 / Rattus norvegicus    Q62765
• Recombinant Spike glycoprotein (HEK293) - DRAFT DATA / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• Kidney (UBERON_0002113)   HEK293-EBNA (CVCL_6974)  
• Mammary Gland (UBERON_0001911)  
• HEK293 (CVCL_0045)  

Reported structure

• N-Linked / Complex / Hex(?1-?)HexNAc(?1-?)[Hex(?1-?)HexNAc(?1-?)]Man(a1-3)[Hex(?1-?)HexNAc(?1-?)[Hex(?1-?)HexNAc(?1-?)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-?)]GlcNAc+"+ HexNAc + (2 x NeuAc)"

Composition

• Hex:7 HexNAc:7 dHex:1 NeuAc:2 (avg mass : 3304.0359 )

Disease

• Cancer, breast (DOID:1612)
• COVID-19 (DOID:0080600)

Reference

• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Unreviewed
• Deducing the N- and O- glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 (2020 - Asif Shajahan, Nitin T. Supekar, Anne S. Gleinich, Parastoo Azadi) / Status : Unreviewed
• Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides (2018 - Yingwei Hu, Punit Shah, David J. Clark, Minghui Ao, Hui Zhang) / Status : Unreviewed
• Structural characterization of recombinant soluble rat neuroligin 1: mapping of secondary structure and glycosylation by mass spectrometry. (2004 - Hoffman RC, Jennings LL, Tsigelny I, Comoletti D, Flynn RE, Sudhof TC, Taylor P) / Status : Reviewed

Reported glycosite

• Alpha-1-acid glycoprotein 1 / Homo sapiens
  •        Asn-72
• Alpha-1-acid glycoprotein 2 / Homo sapiens
  •        Asn-72
• Lumican / Homo sapiens
  •        Asn-88
• Neuroligin 1 / Rattus norvegicus
  •        Undefined site
• Recombinant Spike glycoprotein (HEK293) - DRAFT DATA / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-122
  •        Asn-149
  •        Asn-165
  •        Asn-234
  •        Asn-282
  •        Asn-331
  •        Asn-602
  •        Asn-657
  •        Asn-1098
  •        Asn-1135
  •        Asn-1159

Mass spectrometry observed peptide

• SVQEIQATFFYFTPNKTEDTIFLR (24aa)
  •    P19652    Asn-72
• SVQEIQATFFYFTPNKTEDTIFLR (24aa)
  •    P02763    Asn-72
• AFENVTDLQWLILDHNLLENSK (22aa)
  •    P51884    Asn-88
• TQSLLIVNNATNVVIK (16aa)
  •    P0DTC2    Asn-122
• FQFCNDPFLGVYYHKNNK (18aa)
  •    P0DTC2    Asn-149
• VYSSANNCTFE (11aa)
  •    P0DTC2    Asn-165
• LVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPR (48aa)
  •    P0DTC2    Asn-234
• NGTITDAVDCALDPLSE (17aa)
  •    P0DTC2    Asn-282
• YNENGTITDAVDCALDPLSETK (22aa)
  •    P0DTC2    Asn-282
• FPNITNLCPFGE (12aa)
  •    P0DTC2    Asn-331
• DPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQL (52aa)
  •    P0DTC2    Asn-602
• AGCLIGAEHVNNSYE (15aa)
  •    P0DTC2    Asn-657
• AGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR (36aa)
  •    P0DTC2    Asn-657
• NGTHWFVTQR (10aa)
  •    P0DTC2    Asn-1098
• NCDVVIGIVNNTVYDPLQPELDSFK (25aa)
  •    P0DTC2    Asn-1135
• NHTSPDVDLGDISGINA (17aa)
  •    P0DTC2    Asn-1159
• NHTSPDVDLGDISGIN (16aa)
  •    P0DTC2    Asn-1159