Hex:9 HexNAc:9 dHex:1

Taxonomy

• Homo sapiens (Human)
• Mus musculus (House mouse)
• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Erythropoietin / Homo sapiens    P01588
• Prostaglandin-h2 d-isomerase / Homo sapiens    P41222
• Uromodulin / Homo sapiens    P07911
• WAP four-disulfide core domain protein 2 / Homo sapiens    Q14508
• Lysosome-associated membrane glycoprotein 1 / Mus musculus    P11438
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• Brain (UBERON_0000955)  
• Urine (UBERON_0001088)  
• HEK293 (CVCL_0045)  
• RPMI-1788 (CVCL_2710)   B-Lymphocyte (CL_0000236)  

Reported structure

• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)[Gal(?1-?)GlcNAc(?1-?)]Man(a1-3)[Gal(?1-?)GlcNAc(?1-?)[Gal(?1-?)GlcNAc(?1-?)]Man(a1-6)][GlcNAc(?1-4)]Man(b1-4)GlcNAc(b1-4)[Fuc(?1-6)]GlcNAc+"+ 2 x Gal(?1-?)GlcNAc(?1-?)"

Composition

• Hex:9 HexNAc:9 dHex:1 (avg mass : 3452.1949 )

Disease

• COVID-19 (DOID:0080600)
• Prostate cancer (DOID:10283)

Reference

• Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry (2020 - Yong Zhang, Wanjun Zhao, Yonghong Mao, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Xiaofeng Lu, Jingqiu Cheng, Hao Yang) / Status : Unreviewed
• Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis (2019 - Nicholas M. Riley, Alexander S. Hebert, Michael S. Westphall & Joshua J. Coon ) / Status : Unreviewed
• Distinct urinary glycoprotein signatures in prostate cancer patients (2018 - Rebeca Kawahara, Fabio Ortega, Livia Rosa-Fernandes, Vanessa Guimarães, Daniel Quina, Willian Nahas, Veit Schwämmle, Miguel Srougi, Katia R.M. Leite, Morten Thaysen-Andersen, Martin R. Larsen and Giuseppe Palmisano) / Status : Unreviewed
• Unusual N-glycosylation of a recombinant human erythropoietin expressed in a human lymphoblastoid cell line does not alter its biological properties. (2000 - Cointe D, Bliard R, Jorieux S, Leroy Y, Glacet A, Verbert A, Bourel D, Chirat F) / Status : Reviewed

Reported glycosite

• Erythropoietin / Homo sapiens
  •        Asn-51
  •        Asn-65
  •        Asn-110
• Prostaglandin-h2 d-isomerase / Homo sapiens
  •        Asn-78
• Uromodulin / Homo sapiens
  •        Asn-513
• WAP four-disulfide core domain protein 2 / Homo sapiens
  •        Asn-44
• Lysosome-associated membrane glycoprotein 1 / Mus musculus
  •        Asn-78
• Recombinant Spike glycoprotein (HEK293) / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Asn-122
  •        Asn-282
  •        Asn-657

Mass spectrometry observed peptide

• TGVCPELQADQNCTQECVSDSECADNLK (28aa)
  •    Q14508    Asn-44
• SVVAPATDGGLNLTSTFLR (19aa)
  •    P41222    Asn-78
• ENVSDPSLTITFGR (14aa)
  •    P11438    Asn-78
• TQSLLIVNNATNVVIK (16aa)
  •    P0DTC2    Asn-122
• NGTITDAVDCALDPLSE (17aa)
  •    P0DTC2    Asn-282
• YNENGTITDAVDCALDPLSETK (22aa)
  •    P0DTC2    Asn-282
• FALLMTNCYATPSSNATDPLK (21aa)
  •    P07911    Asn-513
• AGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR (36aa)
  •    P0DTC2    Asn-657
• AGCLIGAEHVNNSYE (15aa)
  •    P0DTC2    Asn-657