Cell line NRK

Taxonomy

• Friend spleen focus-forming virus (anemia inducing strain)

Protein

• Gp55 / Friend spleen focus-forming virus (anemia inducing strain)

Source

• NRK (CVCL_3758)   Kidney (UBERON_0002113)   Fibroblast (CL_0000057)  

Reported structure

• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ 2 x Man(a1-2)"
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ Man(a1-2)"
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ 4 x Man(a1-2)"
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ 3 x Man(a1-2)"
• N-Linked / High-Mannose / Glc(?1-3)Man(a1-2)Man(a1-2)Man(a1-3)[Man(a1-3)[Man(a1-6)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ Man(a1-2)"
• N-Linked / High-Mannose / Glc(?1-3)Man(a1-2)Man(a1-2)Man(a1-3)[Man(a1-3)[Man(a1-6)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Glc(?1-3)Man(a1-2)Man(a1-2)Man(a1-3)[Man(a1-3)[Man(a1-6)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc+"+ 2 x Man(a1-2)"

Composition

• Hex:10 HexNAc:2 (avg mass : 2045.8293 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:9 HexNAc:2 (avg mass : 1883.6869 )
• Hex:7 HexNAc:2 (avg mass : 1559.4021 )

Disease

Reported glycosite

• Gp55 / Friend spleen focus-forming virus (anemia inducing strain) :
  •        Undefined site

Mass spectrometry validated peptide