Cell line HeLa

Taxonomy

• Homo sapiens (Human)
• Human adenovirus type 2
• Human adenovirus type 5

Protein

• Actin-binding LIM protein 1 / Homo sapiens    O14639
• Ataxin-2-like protein / Homo sapiens    Q8WWM7
• BRCA2-interacting transcriptional repressor EMSY / Homo sapiens    Q7Z589
• CDKN2A-interacting protein / Homo sapiens    Q9NXV6
• Cyclin-dependent kinase 12 / Homo sapiens    Q9NYV4
• E3 SUMO-protein ligase RanBP2 / Homo sapiens    P49792
• Early growth response protein 1 / Homo sapiens    P18146
• Eukaryotic translation initiation factor 4 gamma 1 / Homo sapiens    Q04637
• F-actin-capping protein subunit alpha-3 / Homo sapiens    Q96KX2
• Glucocorticoid modulatory element-binding protein 2 / Homo sapiens    Q9UKD1
• Glutamine and serine-rich protein 1 / Homo sapiens    Q2KHR3
• Host Cell Factor 1 / Homo sapiens    P51610
• KAT8 regulatory NSL complex subunit 3 / Homo sapiens    Q9P2N6
• Keratin, type II cytoskeletal 8 / Homo sapiens    P05787
• MAX gene-associated protein / Homo sapiens    Q8IWI9
• Microtubule-associated protein tau / Homo sapiens    P10636
• Msx2-interacting protein / Homo sapiens    Q96T58
• Myocyte-specific enhancer factor 2D / Homo sapiens    Q14814
• Neuroblast differentiation-associated protein AHNAK / Homo sapiens    Q09666
• Nuclear envelope pore membrane protein POM 121 / Homo sapiens    Q96HA1
• Nuclear factor related to kappa-B-binding protein / Homo sapiens    Q6P4R8
• Nuclear mitotic apparatus protein 1 / Homo sapiens    Q14980
• Nuclear pore complex protein Nup153 / Homo sapiens    P49790
• Nuclear pore complex protein Nup214 / Homo sapiens    P35658
• Nuclear pore complex protein Nup98-Nup96 isoform 4 / Homo sapiens    P52948-4
• Nuclear receptor corepressor 1 / Homo sapiens    O75376
• Nucleoporin p62 / Homo sapiens    P37198
• Nucleoprotein TPR / Homo sapiens    P12270
• Nucleosome-remodeling factor subunit BPTF / Homo sapiens    Q12830
• PDZ and LIM domain protein 7 isoform 4 / Homo sapiens    Q9NR12-4
• Perilipin-4 / Homo sapiens    Q96Q06
• Pleckstrin homology domain containing, family A member 5 / Homo sapiens    Q9HAU0
• Pogo transposable element with ZNF domain isoform 2 / Homo sapiens    Q7Z3K3-2
• Polyhomeotic-like protein 3 / Homo sapiens    Q8NDX5
• Prelamin-A/C isoform 3 / Homo sapiens    P02545-3
• Protein EMSY / Homo sapiens    Q7Z589
• Protein lin-54 homolog / Homo sapiens    Q6MZP7
• Protein PRRC2C / Homo sapiens    Q9Y520
• Protein SON / Homo sapiens    P18583-1
• Protein strawberry notch homolog 1 / Homo sapiens    A3KN83
• RanBP2-like and GRIP domain-containing protein 4 / Homo sapiens    Q7Z3J3
• Ras-associated and pleckstrin homology domains-containing protein 1 / Homo sapiens    Q70E73
• Regulation of nuclear pre-mRNA domain-containing protein 2 / Homo sapiens    Q5VT52
• Ribosomal RNA processing protein 1 homolog B / Homo sapiens    Q14684
• RNA binding motif protein 27 / Homo sapiens    Q9P2N5
• RNA-binding protein 14 / Homo sapiens    Q96PK6
• Serine/arginine repetitive matrix protein 2 / Homo sapiens    Q9UQ35
• Serum response factor (srf) / Homo sapiens    P11831
• Synaptopodin / Homo sapiens    Q8N3V7
• TBP-associated factor 4 / Homo sapiens    O00268
• Transcription factor sp1 / Homo sapiens    P08047
• Transcriptional repressor p66-beta / Homo sapiens    Q8WXI9
• Ubiquitin-associated protein 2-like / Homo sapiens    Q14157
• Vimentin / Homo sapiens    P08670
• YTH domain-containing family protein 1 / Homo sapiens    Q9BYJ9
• YTH domain-containing family protein 3 / Homo sapiens    Q7Z739
• Zinc finger CCCH domain-containing protein 14 isoform 2 / Homo sapiens    Q6PJT7-2
• Zinc finger protein 281 / Homo sapiens    Q9Y2X9
• Zinc finger protein 40 / Homo sapiens    P15822
• Zinc finger RNA-binding protein / Homo sapiens    Q96KR1
• Zinc fingers and homeoboxes protein 1 / Homo sapiens    Q9UKY1
• Eg / Human adenovirus type 2
• Fiber protein / Human adenovirus type 2    P03275
• Lg / Human adenovirus type 2
• Fiber protein / Human adenovirus type 5    P11818

Source

• Uterine Cervix (UBERON_0000002)   HeLa (CVCL_0030)   Fibroblast (CL_0000057)  
• Uterine Cervix (UBERON_0000002)   HeLa (CVCL_0030)  
• HeLa (CVCL_0030)  

Reported structure

• N-Linked/O-Linked / Truncated If N-Linked / GlcNAc
• O-Linked / Undefined core / GlcNAc(b

Composition

• HexNAc:1 (avg mass : 221.2103 )

Disease

• Carcinoma (DOID:305)

Reference

• Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis (2010 - Zihao Wang, Namrata D Udeshi, Chad Slawson, Philip D Compton, Kaoru Sakabe, Win D Cheung, Jeffrey Shabanowitz, Donald F Hunt, Gerald W Hart) / Status : Reviewed
• An evaluation of sialation of the nucleoporin p62. (1998 - Fang B, Hanover J, Miller M) / Status : Reviewed
• Identification of O-GlcNAc attachment sites in transcription factors. (1991 - Reason A, Dell A, Morris H, Panico M, Treisman R, Marais R, Hart G, Haltiwanger R) / Status : Reviewed
• Relative accessibility of N-acetylglucosamine in trimers of the adenovirus types 2 and 5 fiber proteins. (1990 - Mullis K, Haltiwanger R, Hart G, Marchase R, Engler J) / Status : Reviewed
• O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation. (1988 - Jackson S, Tjian R) / Status : Reviewed
• The polypeptides of adenovirus. VI. Early and late glycopolypeptides. (1974 - Ishibashi M, Maizel J) / Status : Reviewed

Reported glycosite

• Actin-binding LIM protein 1 / Homo sapiens
  •        Ser-452
• Ataxin-2-like protein / Homo sapiens
  •        Ser-678
  •        Ser-684
• BRCA2-interacting transcriptional repressor EMSY / Homo sapiens
  •        Ser-237
• CDKN2A-interacting protein / Homo sapiens
  •        Thr-322
  •        Ser-349
  •        Thr-379
• Cyclin-dependent kinase 12 / Homo sapiens
  •        Thr-592
  •        Ser-597
• E3 SUMO-protein ligase RanBP2 / Homo sapiens
  •        Thr-1399
• Early growth response protein 1 / Homo sapiens
  •        Thr-119
• Eukaryotic translation initiation factor 4 gamma 1 / Homo sapiens
  •        Ser-61
• F-actin-capping protein subunit alpha-3 / Homo sapiens
  •        Thr-2
• Glucocorticoid modulatory element-binding protein 2 / Homo sapiens
  •        Thr-404
• Glutamine and serine-rich protein 1 / Homo sapiens
  •        Thr-104
  •        Ser-105
• Host Cell Factor 1 / Homo sapiens
  •        Thr-490
  •        Ser-562
  •        Ser-563
  •        Thr-579
  •        Ser-685
  •        Thr-694
  •        Thr-726
  •        Thr-739
  •        Thr-760
  •        Thr-801
  •        Ser-806
  •        Thr-808
  •        Thr-861
  •        Ser-1150
  •        Thr-1239
• KAT8 regulatory NSL complex subunit 3 / Homo sapiens
  •        Thr-860
• Keratin, type II cytoskeletal 8 / Homo sapiens
  •        Ser-13
  •        Ser-15
• MAX gene-associated protein / Homo sapiens
  •        Ser-1557
• Microtubule-associated protein tau / Homo sapiens
  •        Ser-489
• Msx2-interacting protein / Homo sapiens
  •        Thr-2529
  •        Ser-2530
  •        Ser-2811
  •        Thr-2924
• Myocyte-specific enhancer factor 2D / Homo sapiens
  •        Ser-275
• Neuroblast differentiation-associated protein AHNAK / Homo sapiens
  •        Ser-5603
• Nuclear envelope pore membrane protein POM 121 / Homo sapiens
  •        Thr-693
  •        Ser-694
  •        Ser-738
• Nuclear factor related to kappa-B-binding protein / Homo sapiens
  •        Thr-750
  •        Thr-841
• Nuclear mitotic apparatus protein 1 / Homo sapiens
  •        Ser-1844
• Nuclear pore complex protein Nup153 / Homo sapiens
  •        Ser-534
  •        Ser-544
  •        Ser-908
  •        Ser-909
  •        Thr-1112
  •        Thr-1156
  •        Thr-1179
  •        Thr-1180
• Nuclear pore complex protein Nup214 / Homo sapiens
  •        Thr-580
  •        Ser-1056
  •        Thr-1201
  •        Ser-1354
• Nuclear pore complex protein Nup98-Nup96 isoform 4 / Homo sapiens
  •        Thr-184
  •        Thr-567
  •        Thr-568
• Nuclear receptor corepressor 1 / Homo sapiens
  •        Ser-1487
• Nucleoporin p62 / Homo sapiens
  •        Undefined site
• Nucleoprotein TPR / Homo sapiens
  •        Ser-1676
• Nucleosome-remodeling factor subunit BPTF / Homo sapiens
  •        Ser-1749
  •        Thr-1753
  •        Ser-2081
  •        Thr-2094
  •        Thr-2103
• PDZ and LIM domain protein 7 isoform 4 / Homo sapiens
  •        Ser-89
• Perilipin-4 / Homo sapiens
  •        Ser-704
• Pleckstrin homology domain containing, family A member 5 / Homo sapiens
  •        Ser-382
• Pogo transposable element with ZNF domain isoform 2 / Homo sapiens
  •        Ser-191
• Polyhomeotic-like protein 3 / Homo sapiens
  •        Thr-283
• Prelamin-A/C isoform 3 / Homo sapiens
  •        Ser-582
  •        Thr-613
• Protein EMSY / Homo sapiens
  •        Ser-228
  •        Ser-236
  •        Thr-501
  •        Thr-506
  •        Thr-534
  •        Thr-535
  •        Ser-557
  •        Thr-1120
• Protein lin-54 homolog / Homo sapiens
  •        Ser-238
• Protein PRRC2C / Homo sapiens
  •        Thr-2243
  •        Thr-2694
• Protein SON / Homo sapiens
  •        Thr-252
  •        Thr-253
• Protein strawberry notch homolog 1 / Homo sapiens
  •        Thr-112
• RanBP2-like and GRIP domain-containing protein 4 / Homo sapiens
  •        Ser-919
• Ras-associated and pleckstrin homology domains-containing protein 1 / Homo sapiens
  •        Thr-784
• Regulation of nuclear pre-mRNA domain-containing protein 2 / Homo sapiens
  •        Ser-399
  •        Thr-400
• Ribosomal RNA processing protein 1 homolog B / Homo sapiens
  •        Ser-731
• RNA binding motif protein 27 / Homo sapiens
  •        Ser-546
  •        Ser-738
• RNA-binding protein 14 / Homo sapiens
  •        Ser-244
  •        Ser-254
  •        Ser-256
  •        Ser-280
• Serine/arginine repetitive matrix protein 2 / Homo sapiens
  •        Ser-2236
• Serum response factor (srf) / Homo sapiens
  •        Ser-277
  •        Ser-316
• Synaptopodin / Homo sapiens
  •        Ser-165
• TBP-associated factor 4 / Homo sapiens
  •        Ser-528
• Transcription factor sp1 / Homo sapiens
  •        Undefined site
• Transcriptional repressor p66-beta / Homo sapiens
  •        Ser-584
• Ubiquitin-associated protein 2-like / Homo sapiens
  •        Thr-782
  •        Thr-783
• Vimentin / Homo sapiens
  •        Ser-7
  •        Thr-33
  •        Ser-34
• YTH domain-containing family protein 1 / Homo sapiens
  •        Ser-196
• YTH domain-containing family protein 3 / Homo sapiens
  •        Thr-205
• Zinc finger CCCH domain-containing protein 14 isoform 2 / Homo sapiens
  •        Thr-369
• Zinc finger protein 281 / Homo sapiens
  •        Ser-891
• Zinc finger protein 40 / Homo sapiens
  •        Thr-2325
• Zinc finger RNA-binding protein / Homo sapiens
  •        Ser-148
  •        Thr-149
  •        Ser-195
  •        Thr-202
• Zinc fingers and homeoboxes protein 1 / Homo sapiens
  •        Ser-450
• Eg / Human adenovirus type 2
  •        Undefined site
• Fiber protein / Human adenovirus type 2
  •        Undefined site
• Lg / Human adenovirus type 2
  •        Undefined site
• Fiber protein / Human adenovirus type 5
  •        Undefined site

Mass spectrometry observed peptide

• TLSVLSR (7aa)
  •    Q96KX2    Thr-2
• SVSSSSYR (8aa)
  •    P08670    Ser-7
• VSTSGPR (7aa)
  •    P05787    Ser-15
• VSTSGPR (7aa)
  •    P05787    Ser-13
• SYVTTSTR (8aa)
  •    P08670    Ser-34
• SYVTTSTR (8aa)
  •    P08670    Thr-33
• AQPPSSAASR (10aa)
  •    Q04637    Ser-61
• AQPVQSK (7aa)
  •    Q9NR12-4    Ser-89
• TSQGTVPTALAFER (14aa)
  •    Q2KHR3    Thr-104
• TSQGTVPTALAFER (14aa)
  •    Q2KHR3    Ser-105
• TPPVTTNR (8aa)
  •    A3KN83    Thr-112
• VLVETSYPSQTTR (13aa)
  •    P18146    Thr-119
• STAPAVAYDSK (11aa)
  •    Q96KR1    Ser-148
• STAPAVAYDSK (11aa)
  •    Q96KR1    Thr-149
• VSTPATTTSTFSR (13aa)
  •    Q8N3V7    Ser-165
• AGVSTNISTK (10aa)
  •    P52948-4    Thr-184
• AGYSQGATQYTQAQQTR (17aa)
  •    Q96KR1    Ser-195
• AGYSQGATQYTQAQQTR (17aa)
  •    Q96KR1    Thr-202
• IGDVSSSAVK (10aa)
  •    Q9BYJ9    Ser-196
• IGGDLTAAVTK (11aa)
  •    Q7Z739    Thr-205
• AVVPVSK (7aa)
  •    Q7Z589    Ser-228
• TITVPVSGSPK (11aa)
  •    Q7Z589    Ser-236
• PSTQTTNTTTQK (12aa)
  •    Q7Z589    Ser-237
• TPTSGPVITK (10aa)
  •    Q6MZP7    Ser-238
• AQPSVSLGAAYR (12aa)
  •    Q96PK6    Ser-244
• ILDSFAAAPVPTTTLVLK (18aa)
  •    P18583-1    Thr-253
• ILDSFAAAPVPTTTLVLK (18aa)
  •    P18583-1    Thr-252
• AQPSASLGVGYR (12aa)
  •    Q96PK6    Ser-256
• AQPSASLGVGYR (12aa)
  •    Q96PK6    Ser-254
• VITSQAGK (8aa)
  •    Q14814    Ser-275
• AQPSVSLGAPYR (12aa)
  •    Q96PK6    Ser-280
• STAVTR (6aa)
  •    Q8NDX5    Thr-283
• TSSEASVSSSVAK (13aa)
  •    Q9NXV6    Thr-322
• SSSSTNTSLLTSK (13aa)
  •    Q9NXV6    Ser-349
• SSTNTSLLTS (10aa)
  •    Q9NXV6    Ser-349
• TTNYSTVPQK (10aa)
  •    Q6PJT7-2    Thr-369
• SSSQTSGSLVSK (12aa)
  •    Q9NXV6    Thr-379
• IVNVSLADLR (10aa)
  •    Q9HAU0    Ser-382
• SAVSTSVPTKPTENISK (17aa)
  •    Q5VT52    Ser-399
• SAVSTSVPTKPTENISK (17aa)
  •    Q5VT52    Thr-400
• VVSTLPSTVLGK (12aa)
  •    Q9UKD1    Thr-404
• PATAAVPTSQSVK (13aa)
  •    Q9UKY1    Ser-450
• STSQGSINSPVYSR (14aa)
  •    O14639    Ser-452
• PVVSSAGTTSDK (12aa)
  •    P10636    Ser-489
• VTGPQATGTPLVTMRPASQAGK (22aa)
  •    P51610    Thr-490
• LVTTPTGTQATYTRPTVSPSIGR (23aa)
  •    Q7Z589    Thr-501
• LVTTPTGTQATYTRPTVSPSIGR (23aa)
  •    Q7Z589    Thr-506
• QVSQAQTTVQPSATLQR (17aa)
  •    O00268    Ser-528
• TTSGSIITVVPK (12aa)
  •    Q7Z589    Thr-535
• TTSGSIITVVPK (12aa)
  •    Q7Z589    Thr-534
• STEANVLPPSSIGFTFSVPVAK (22aa)
  •    P49790    Ser-534
• STEANVLPPSSIGFTFSVPVAK (22aa)
  •    P49790    Ser-544
• AANIVIQTEPPVPVSINSNITR (22aa)
  •    Q9P2N5    Ser-546
• IISSNIVSGTTTK (13aa)
  •    Q7Z589    Ser-557
• IPPSSAPTVLSVPAGTTIVK (20aa)
  •    P51610    Ser-563
• IPPSSAPTVLSVPAGTTIVK (20aa)
  •    P51610    Ser-562
• ALQTTGTAK (9aa)
  •    P52948-4    Thr-568
• ALQTTGTAK (9aa)
  •    P52948-4    Thr-567
• TMAVTPGTTTLPATVK (16aa)
  •    P51610    Thr-579
• PSFPPSTSAVK (11aa)
  •    P35658    Thr-580
• SVGGSGGGSFGDNLVTR (17aa)
  •    P02545-3    Ser-582
• SISQSISGQK (10aa)
  •    Q8WXI9    Ser-584
• TSAVSSQANSQPPVQVSVK (19aa)
  •    Q9NYV4    Thr-592
• TSAVSSQANSQPPVQVSVK (19aa)
  •    Q9NYV4    Ser-597
• ASASGSGAQVGGPISSGSSASSVTVTR (27aa)
  •    P02545-3    Thr-613
• STSTPTSPGPR (11aa)
  •    Q8WWM7    Ser-684
• STSTPTSPGPR (11aa)
  •    Q8WWM7    Ser-678
• VMSVVQTKPVQTSAVTGQASTGPVTQIIQTK (31aa)
  •    P51610    Ser-685
• APPTLQAETATKPQATSAPSPAPK (24aa)
  •    Q96HA1    Thr-693
• PQATSAPSPAPK (12aa)
  •    Q96HA1    Ser-694
• PVQTSAVTGQASTGPVTQIIQTK (23aa)
  •    P51610    Thr-694
• GTVQTSVDTTK (11aa)
  •    Q96Q06    Ser-704
• LVTSADGK (8aa)
  •    P51610    Thr-726
• TPTSSPASSPLVAK (14aa)
  •    Q14684    Ser-731
• PQTSGAYVLNK (11aa)
  •    Q9P2N5    Ser-738
• PIFTAPPKSEK (11aa)
  •    Q96HA1    Ser-738
• PTTIITTTQASGAGTK (16aa)
  •    P51610    Thr-739
• SGPSTVSEPAK (11aa)
  •    Q6P4R8    Thr-750
• PTILGISSVSPSTTKPGTTTIIK (23aa)
  •    P51610    Thr-760
• SSVATTSGK (9aa)
  •    Q14157    Thr-782
• SSVATTSGK (9aa)
  •    Q14157    Thr-783
• PLVtIPAPTSTK (12aa)
  •    Q70E73    Thr-784
• SPITIITTKVMTSGTGAPAK (20aa)
  •    P51610    Thr-801
• VMTSGTGAPAK (11aa)
  •    P51610    Thr-808
• VMTSGTGAPAK (11aa)
  •    P51610    Ser-806
• VPATATQTK (9aa)
  •    Q6P4R8    Thr-841
• VPTTITLTLR (10aa)
  •    Q9P2N6    Thr-860
• LVTPVTVSAVKPAVTTLVVK (20aa)
  •    P51610    Thr-861
• TPTSQSYR (8aa)
  •    Q9Y2X9    Ser-891
• FGVSSSSSGPSQTLTSTGNFK (21aa)
  •    P49790    Ser-909
• FGVSSSSSGPSQTLTSTGNFK (21aa)
  •    P49790    Ser-908
• EGFSIPVSADGFK (13aa)
  •    Q7Z3J3    Ser-919
• SHLVHGSSPGVMGTSVAtsAs (21aa)
  •    P35658    Ser-1056
• SHLVHGSSPGVM-oxGTSVATSASK (25aa)
  •    P35658    Ser-1056
• QQEPVTSTSLVFGK (14aa)
  •    P49790    Thr-1112
• ITGGSSVPK (9aa)
  •    Q7Z589    Thr-1120
• ISVATGALEAAQGSK (15aa)
  •    P51610    Ser-1150
• STFSFSM-oxTKPSEK (16aa)
  •    P49790    Thr-1156
• ATFAFGAQTSTTADQGAAK (19aa)
  •    P49790    Thr-1180
• ATFAFGAQTSTTADQGAAK (19aa)
  •    P49790    Thr-1179
• IETAVTSTPSASGQFSK (17aa)
  •    P35658    Thr-1201
• HSHAVSTAAMTR (12aa)
  •    P51610    Thr-1239
• ASSTSLTSTQPTK (13aa)
  •    P35658    Ser-1354
• TSPENVQDRFALVTPK (16aa)
  •    P49792    Thr-1399
• TPVSYQNTMSR (11aa)
  •    O75376    Ser-1487
• IPGVSTPQTLAGTQK (15aa)
  •    Q8IWI9    Ser-1557
• GIASTSDPPTANIKPTPVVSTPSK (24aa)
  •    P12270    Ser-1676
• STVTTTTTTVTK (12aa)
  •    Q12830    Thr-1753
• STVTTTTTTVTK (12aa)
  •    Q12830    Ser-1749
• SAPASQASLR (10aa)
  •    Q14980    Ser-1844
• VM-oxVAPISGSVTTGTK (18aa)
  •    Q12830    Ser-2081
• MVLTTK (6aa)
  •    Q12830    Thr-2094
• VGSPATVTFQQNK (13aa)
  •    Q12830    Thr-2103
• IPAASAAAMNLASAR (15aa)
  •    Q9UQ35    Ser-2236
• RETIQQSSSLTSVPPTTFSLTFK (23aa)
  •    Q9Y520    Thr-2243
• AVAITQSPSSVR (12aa)
  •    P15822    Thr-2325
• ASDVDTSSSTLR (12aa)
  •    Q96T58    Thr-2529
• ASDVDTSSSTLR (12aa)
  •    Q96T58    Ser-2530
• ATSTSPNSQSSK (12aa)
  •    Q9Y520    Thr-2694
• VNTSEGVVLLSYSGQK (16aa)
  •    Q96T58    Ser-2811
• ADRPSLEKPEPIHLSVSTPVTQGGTVK (27aa)
  •    Q96T58    Thr-2924
• GPQVSSALNLDTSK (14aa)
  •    Q09666    Ser-5603