Cell line HEK293-F

Taxonomy

• Homo sapiens (Human)
• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens    P01860
• Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens    P01860
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) / Homo sapiens    P01857
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant A121N / Homo sapiens    P01857
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant L115N / Homo sapiens    P01857
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant L177N / Homo sapiens    P01857
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant L182N / Homo sapiens    P01857
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant Q178N / Homo sapiens    P01857
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant T198N / Homo sapiens    P01857
• Immunoglobulin kappa light chain (Trastuzumab) mutant Q160N / Homo sapiens    P0DOX7
• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• HEK293-F (CVCL_6642)  

Reported structure

• N-Linked / Complex / GlcNAc(b1-2)Man(a1-3)[GlcNAc(b1-2)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / GlcNAc(b1-2)Man(a1-3)[GlcNAc(b1-2)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Complex / GlcNAc(b1-2)[GlcNAc(b1-4)]Man(a1-6)[GlcNAc(b1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Complex / Structure 11110
• N-Linked / Complex / Structure 11227
• N-Linked / Complex / Gal(b1-4)GlcNAc(b1-2)Man(a1-3)[Gal(b1-4)GlcNAc(b1-2)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Complex / Structure 11124
• N-Linked / Complex / Structure 10835
• N-Linked / Complex / Structure 11152
• N-Linked / Complex / Structure 11154
• N-Linked / Complex / Structure 11123
• N-Linked / Complex / Structure 11253
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Man(a1-2)Man(a1-6)[Man(a1-3)]Man(a1-6)[Man(a1-2)Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Man(a1-2)Man(a1-3)[Man(a1-2)Man(a1-6)]Man(a1-6)[Man(a1-2)Man(a1-2)Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Hybrid / Structure 9591
• N-Linked / Hybrid / Gal(b1-4)GlcNAc(b1-2)Man(a1-3)[Man(a1-3)[Man(a1-6)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / Hybrid / Structure 11125
• N-Linked / Hybrid / Structure 11109
• N-Linked / Hybrid / NeuAc(a2-?)Gal(b1-4)GlcNAc(b1-2)Man(a1-3)[Man(a1-3)[Man(a1-6)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• O-Linked / Core 0 / GalNAc
• O-Linked / Core 1 / Gal(b1-3)GalNAc
• O-Linked / Core 1 / Gal(b1-3)GalNAc(a + "Neu5Ac(a"
• O-Linked / Core 1 / Structure 10127
• O-Linked / Core 1 / Structure 10140

Composition

• HexNAc:1 (avg mass : 221.2103 )
• Hex:1 HexNAc:1 (avg mass : 383.3527 )
• Hex:1 HexNAc:1 NeuAc:1 (avg mass : 674.6106 )
• Hex:1 HexNAc:1 NeuAc:2 (avg mass : 965.8685 )
• Hex:2 HexNAc:2 NeuAc:2 (avg mass : 1331.2059 )
• Hex:3 HexNAc:4 (avg mass : 1317.2225 )
• Hex:3 HexNAc:4 dHex:1 (avg mass : 1463.3655 )
• Hex:3 HexNAc:5 (avg mass : 1520.4175 )
• Hex:4 HexNAc:4 dHex:1 (avg mass : 1625.5079 )
• Hex:4 HexNAc:5 (avg mass : 1682.5599 )
• Hex:4 HexNAc:5 dHex:1 (avg mass : 1828.7029 )
• Hex:4 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2119.9608 )
• Hex:4 HexNAc:5 dHex:2 (avg mass : 1974.8459 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:5 HexNAc:3 NeuAc:1 (avg mass : 1729.5702 )
• Hex:5 HexNAc:3 dHex:1 (avg mass : 1584.4553 )
• Hex:5 HexNAc:4 NeuAc:1 (avg mass : 1932.7652 )
• Hex:5 HexNAc:4 dHex:1 (avg mass : 1787.6503 )
• Hex:5 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 2078.9082 )
• Hex:5 HexNAc:5 dHex:1 (avg mass : 1990.8453 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:6 HexNAc:3 (avg mass : 1600.4547 )
• Hex:6 HexNAc:3 NeuAc:1 (avg mass : 1891.7126 )
• Hex:6 HexNAc:3 dHex:1 (avg mass : 1746.5977 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )
• Hex:9 HexNAc:2 (avg mass : 1883.6869 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed
• Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity. (2021 - Cruz E, Sifniotis V, Sumer-Bayraktar Z, Reslan M, Wilkinson-White L, Cordwell S, Kayser V) / Status : Reviewed
• Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3) (2015 - Rosina Plomp, Gillian Dekkers, Yoann Rombouts, Remco Visser, Carolien A.M.Koeleman, Guinevere S.M.Kammeijer, Bas C.Jansen, Theo Rispens, Paul J.Hensbergen, Gestur Vidarsson, Manfred Wuhrer) / Status : Reviewed

Reported glycosite

• Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens
  •        Thr-122
  •        Thr-137
  •        Thr-152
• Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens
  •        Thr-122
  •        Thr-137
  •        Thr-152
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) / Homo sapiens
  •        Undefined site
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant A121N / Homo sapiens
  •        Undefined site
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant L115N / Homo sapiens
  •        Undefined site
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant L177N / Homo sapiens
  •        Undefined site
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant L182N / Homo sapiens
  •        Undefined site
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant Q178N / Homo sapiens
  •        Undefined site
• Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant T198N / Homo sapiens
  •        Undefined site
• Immunoglobulin kappa light chain (Trastuzumab) mutant Q160N / Homo sapiens
  •        Undefined site
• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-63
  •        Thr-73
  •        Thr-76
  •        Thr-114
  •        Thr-167
  •        Thr-236
  •        Thr-478
  •        Thr-676
  •        Thr-678
  •        Ser-803
  •        Thr-1076
  •        Thr-1077
  •        Ser-1097

Mass spectrometry observed peptide

• NSGALTSGVHTFPAVLQSSGLY (22aa)
  •    P01857        Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant Q178N / Homo sapiens
  •       178:Q→N
  •    P01857        Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant L182N / Homo sapiens
  •       182:L→N
  •    P01857        Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant L177N / Homo sapiens
  •       177:L→N
• SLSSVVTVPSSSLGTQTY (18aa)
  •    P01857        Immunoglobulin heavy constant gamma 1 (Trastuzumab) mutant T198N / Homo sapiens
  •       198:T→N
• FSNVTWFHAIHVSGTNGTK (19aa)
  •    P0DTC2    Thr-63     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAIHVSGTNGTK (12aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAIHVSGTNGTKRF (14aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VSGTNGTKRF (10aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• TQSLLIVNNATNVVIK (16aa)
  •    P0DTC2    Thr-114     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• SCDTPPPCPR (10aa)
  •    P01860    Thr-137     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens
  •    P01860    Thr-122     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens
  •    P01860    Thr-137     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens
  •    P01860    Thr-122     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens
  •    P01860    Thr-152     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens
  •    P01860    Thr-152     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens
• VYSSANNCTFEYVSQPFLMDLEGK (24aa)
  •    P0DTC2    Thr-167     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• SALEPLVDLPIGINIT (16aa)
  •    P0DTC2    Thr-236     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VDLPIGINIT (10aa)
  •    P0DTC2    Thr-236     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• TPCNGVEGFNCY (12aa)
  •    P0DTC2    Thr-478     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• QTQTNSPGSASSVASQ (16aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
  •    P0DTC2    Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
• QTQTNSPGSASSVASQSIIAY (21aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
  •    P0DTC2    Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
• DFGGFNFSQILPDPSK (16aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• AQEKNFTTAPAICHDGKAHFPREGVF (26aa)
  •    P0DTC2    Thr-1076     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1077     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• EGVFVSNGTHW (11aa)
  •    P0DTC2    Ser-1097     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)