Cell line HEK293-F

Taxonomy

• Homo sapiens (Human)
• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens    P01860
• Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens    P01860
• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• HEK293-F (CVCL_6642)  

Reported structure

• O-Linked / Core 0 / GalNAc
• O-Linked / Core 1 / Gal(b1-3)GalNAc
• O-Linked / Core 1 / Gal(b1-3)GalNAc(a + "Neu5Ac(a"
• O-Linked / Core 1 / Structure 10127
• O-Linked / Core 1 / Structure 10140

Composition

• HexNAc:1 (avg mass : 221.2103 )
• Hex:1 HexNAc:1 (avg mass : 383.3527 )
• Hex:1 HexNAc:1 NeuAc:1 (avg mass : 674.6106 )
• Hex:1 HexNAc:1 NeuAc:2 (avg mass : 965.8685 )
• Hex:2 HexNAc:2 NeuAc:2 (avg mass : 1331.2059 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed
• Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3) (2015 - Rosina Plomp, Gillian Dekkers, Yoann Rombouts, Remco Visser, Carolien A.M.Koeleman, Guinevere S.M.Kammeijer, Bas C.Jansen, Theo Rispens, Paul J.Hensbergen, Gestur Vidarsson, Manfred Wuhrer) / Status : Reviewed

Reported glycosite

• Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens
  •        Thr-122
  •        Thr-137
  •        Thr-152
• Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens
  •        Thr-122
  •        Thr-137
  •        Thr-152
• Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-63
  •        Thr-73
  •        Thr-76
  •        Thr-114
  •        Thr-167
  •        Thr-236
  •        Thr-478
  •        Thr-676
  •        Thr-678
  •        Ser-803
  •        Thr-1076
  •        Thr-1077
  •        Ser-1097

Mass spectrometry observed peptide

• FSNVTWFHAIHVSGTNGTK (19aa)
  •    P0DTC2    Thr-63     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAIHVSGTNGTK (12aa)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAIHVSGTNGTKRF (14aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VSGTNGTKRF (10aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• TQSLLIVNNATNVVIK (16aa)
  •    P0DTC2    Thr-114     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• SCDTPPPCPR (10aa)
  •    P01860    Thr-122     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens
  •    P01860    Thr-122     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens
  •    P01860    Thr-137     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens
  •    P01860    Thr-137     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens
  •    P01860    Thr-152     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(g) / monoclonal) / Homo sapiens
  •    P01860    Thr-152     Immunoglobulin gamma-3 (anti-GDob1 allotype G3m(s) / monoclonal) / Homo sapiens
• VYSSANNCTFEYVSQPFLMDLEGK (24aa)
  •    P0DTC2    Thr-167     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• SALEPLVDLPIGINIT (16aa)
  •    P0DTC2    Thr-236     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VDLPIGINIT (10aa)
  •    P0DTC2    Thr-236     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• TPCNGVEGFNCY (12aa)
  •    P0DTC2    Thr-478     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• QTQTNSPGSASSVASQ (16aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
  •    P0DTC2    Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
• QTQTNSPGSASSVASQSIIAY (21aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
  •    P0DTC2    Thr-676     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→GSAS
• DFGGFNFSQILPDPSK (16aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• AQEKNFTTAPAICHDGKAHFPREGVF (26aa)
  •    P0DTC2    Thr-1077     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1076     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• EGVFVSNGTHW (11aa)
  •    P0DTC2    Ser-1097     Recombinant Spike glycoprotein (HEK293-F) - ectodomain trimer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)