Cell line Schneider 2

Taxonomy

• Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)

Protein

• Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2
• Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)    P0DTC2

Source

• Schneider 2 (CVCL_Z232)  

Reported structure

• O-Linked / Core 0 / GalNAc
• O-Linked / Core 1 / Gal(b1-3)GalNAc

Composition

• HexNAc:1 (avg mass : 221.2103 )
• Hex:1 HexNAc:1 (avg mass : 383.3527 )

Disease

• COVID-19 (DOID:0080600)

Reference

• Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells (2021 - Ieva Bagdonaite, Andrew J. Thompson, Xiaoning Wang, Max Søgaard, Cyrielle Fougeroux, Martin Frank, Jolene K. Diedrich, John R. Yates 3rd, Ali Salanti, Sergey Y. Vakhrushev, James C. Paulson, Hans H. Wandall) / Status : Reviewed

Reported glycosite

• Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Undefined site
  •        Thr-63
  •        Ser-71
  •        Ser-71
  •        Thr-73
  •        Thr-76
  •        Ser-94
  •        Thr-95
  •        Thr-124
  •        Ser-151
  •        Thr-478
  •        Thr-618
  •        Thr-676
  •        Thr-678
  •        Ser-803
  •        Thr-1076
  •        Thr-1077
  •        Ser-1097
  •        Thr-1100
• Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •        Thr-315
  •        Thr-323
  •        Thr-333
  •        Thr-345
  •        Thr-415
  •        Thr-523

Mass spectrometry observed peptide

• FSNVTWFHAIHVSGTNGTK (19aa)
  •    P0DTC2        Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• FHAIHVSGTNGTKRF (15aa)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• AIHVSGTNGTKRF (13aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAIHVSGTNGTK (12aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Ser-71     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VTWFHAIHVSGTNGTK (16aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-63     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAIHVSGTNGTKRF (14aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VSGTNGTKRF (10aa)
  •    P0DTC2    Thr-76     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-73     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• ASTEKSNIIRGW (12aa)
  •    P0DTC2    Ser-94     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-95     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• IVNNATNVVIKVCEF (15aa)
  •    P0DTC2    Thr-124     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• YHKNNKSWMESEF (13aa)
  •    P0DTC2    Ser-151     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HKNNKSWMESEF (12aa)
  •    P0DTC2    Ser-151     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• TVEKGIYQTSNF (12aa)
  •    P0DTC2    Thr-315     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVR (10aa)
  •    P0DTC2    Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVRFPNITNL (17aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-323     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• RVQPTESIVRFPNITNLCPF (20aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• GIYQTSNFRVQPTESIVR (18aa)
  •    P0DTC2    Thr-333     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• GEVFNATRFASVY (13aa)
  •    P0DTC2    Thr-345     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NATRFASVY (9aa)
  •    P0DTC2    Thr-345     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• VIRGDEVRQIAPGQTGKI (18aa)
  •    P0DTC2    Thr-415     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• TPCNGVEGFNCY (12aa)
  •    P0DTC2    Thr-478     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• HAPATVCGPKKSTNL (15aa)
  •    P0DTC2    Thr-523     Recombinant Spike glycoprotein (Schneider 2) - Receptor-binding domain monomer / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NCTEVPVAIHADQLTPTWR (19aa)
  •    P0DTC2    Thr-618     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NCTEVPVAIHADQL (14aa)
  •    P0DTC2    Thr-618     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• CDIPIGAGICASYQTQTNSPAR (22aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→ARAA
• QTQTNSPARAA (11aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→ARAA
• QTQTNSPARAASVASQSIIAY (21aa)
  •    P0DTC2    Thr-678     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→ARAA
  •    P0DTC2    Thr-676     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •       682:RRAR→ARAA
• FGGFNFSQILPDPSKPSKR (19aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NFSQILPDPSKPS (13aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NFSQILPDPSKPSKR (15aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• FGGFNFSQILPDPSKPSK (18aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• NFSQILPDPSKPSKRSF (17aa)
  •    P0DTC2    Ser-803     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• KNFTTAPAICHDGK (14aa)
  •    P0DTC2    Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• AQEKNFTTAPAICHDGKAHFPREGVF (26aa)
  •    P0DTC2    Thr-1077     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1076     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• GVFVSNGTHWFVTQR (15aa)
  •    P0DTC2    Ser-1097     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• EGVFVSNGTHWFVTQR (16aa)
  •    P0DTC2    Ser-1097     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
  •    P0DTC2    Thr-1100     Recombinant Spike glycoprotein (Schneider 2) - ectodomain / Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)