Cell line HEK293T

Taxonomy

• Homo sapiens (Human)

Protein

• Adipocyte plasma membrane-associated protein / Homo sapiens    Q9HDC9
• Immunoglobulin epsilon chain c region / Homo sapiens    P01854

Source

• Kidney (UBERON_0002113)   HEK293T (CVCL_0063)  
• HEK293T (CVCL_0063)  

Reported structure

• N-Linked / Complex / Structure 1850
• N-Linked / Complex / Structure 2643
• N-Linked / Complex / Structure 9835
• N-Linked / Complex / Structure 10309
• N-Linked / Complex / Structure 2888
• N-Linked / Complex / Structure 10244
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)Man(a1-3)[Gal(?1-?)GlcNAc(?1-?)Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Complex / Structure 10169
• N-Linked / Complex / Structure 10199
• N-Linked / Complex / Structure 10207
• N-Linked / Complex / Structure 10216
• N-Linked / Complex / Structure 10159
• N-Linked / Complex / Structure 10206
• N-Linked / Complex / Structure 3446
• N-Linked / Complex / Structure 10148
• N-Linked / Complex / Structure 10167
• N-Linked / Complex / Structure 10197
• N-Linked / Complex / Structure 10174
• N-Linked / Complex / Structure 10229
• N-Linked / Complex / Structure 10166
• N-Linked / Complex / Structure 10283
• N-Linked / Complex / Structure 10305
• N-Linked / Complex / Structure 10146
• N-Linked / Complex / Structure 10198
• N-Linked / Complex / Structure 10288
• N-Linked / Complex / Structure 10155
• N-Linked / Complex / Structure 10251
• N-Linked / Complex / Structure 10296
• N-Linked / Complex / Structure 10225
• N-Linked / Complex / Gal(?1-?)GlcNAc(?1-?)[Gal(?1-?)GlcNAc(?1-?)]Man(a1-3)[Gal(?1-?)GlcNAc(?1-?)[Gal(?1-?)GlcNAc(?1-?)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Complex / Structure 10123
• N-Linked / Complex / Structure 10217
• N-Linked / Complex / Structure 10170
• N-Linked / Complex / Structure 10173
• N-Linked / Complex / Structure 10249
• N-Linked / Complex / Structure 10278
• N-Linked / Complex / Structure 10179
• N-Linked / Complex / Structure 10232
• N-Linked / Complex / Structure 10158
• N-Linked / Complex / Structure 10247
• N-Linked / Complex / Structure 10156
• N-Linked / Complex / Structure 10171
• N-Linked / Complex / Structure 10308
• N-Linked / Complex / Structure 10137
• N-Linked / Complex / Structure 10182
• N-Linked / Complex / Structure 10149
• N-Linked / Complex / Structure 10193
• N-Linked / Complex / Structure 10243
• N-Linked / High-Mannose / Man(a1-3)[Man(a1-6)]Man(a1-6)[Man(a1-3)]Man(b1-4)GlcNAc(b1-4)GlcNAc
• N-Linked / High-Mannose / Structure 262
• N-Linked / High-Mannose / Structure 10138
• N-Linked / High-Mannose / Structure 314
• N-Linked / High-Mannose / Structure 979
• N-Linked / High-Mannose / Structure 371
• N-Linked / High-Mannose / Structure 2636
• N-Linked / High-Mannose / Structure 3677
• N-Linked / Hybrid / Structure 834
• N-Linked / Hybrid / Structure 51
• N-Linked / Hybrid / Structure 366
• N-Linked / Hybrid / Structure 1713
• N-Linked / Hybrid / Structure 10267
• N-Linked / Hybrid / Structure 10178
• N-Linked / Hybrid / Structure 10252
• N-Linked / Pauci-Mannose / Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)[Fuc(a1-6)]GlcNAc
• N-Linked / Pauci-Mannose / Structure 1786

Composition

• Hex:3 HexNAc:2 dHex:1 (avg mass : 1056.9755 )
• Hex:3 HexNAc:3 dHex:1 (avg mass : 1260.1705 )
• Hex:3 HexNAc:4 dHex:1 (avg mass : 1463.3655 )
• Hex:3 HexNAc:5 dHex:1 (avg mass : 1666.5605 )
• Hex:3 HexNAc:6 dHex:1 (avg mass : 1869.7555 )
• Hex:3 HexNAc:6 dHex:2 (avg mass : 2015.8985 )
• Hex:4 HexNAc:2 (avg mass : 1072.9749 )
• Hex:4 HexNAc:2 dHex:1 (avg mass : 1219.1179 )
• Hex:4 HexNAc:4 dHex:1 (avg mass : 1625.5079 )
• Hex:4 HexNAc:5 dHex:1 (avg mass : 1828.7029 )
• Hex:4 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2119.9608 )
• Hex:4 HexNAc:5 dHex:2 (avg mass : 1974.8459 )
• Hex:5 HexNAc:2 (avg mass : 1235.1173 )
• Hex:5 HexNAc:2 dHex:1 (avg mass : 1381.2603 )
• Hex:5 HexNAc:3 (avg mass : 1438.3123 )
• Hex:5 HexNAc:3 dHex:1 (avg mass : 1584.4553 )
• Hex:5 HexNAc:3 dHex:1 NeuAc:1 (avg mass : 1875.7132 )
• Hex:5 HexNAc:4 dHex:1 (avg mass : 1787.6503 )
• Hex:5 HexNAc:4 dHex:1 NeuAc:1 (avg mass : 2078.9082 )
• Hex:5 HexNAc:5 dHex:1 (avg mass : 1990.8453 )
• Hex:5 HexNAc:6 dHex:1 (avg mass : 2194.0403 )
• Hex:5 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2485.2982 )
• Hex:6 HexNAc:2 (avg mass : 1397.2597 )
• Hex:6 HexNAc:3 dHex:1 NeuAc:1 (avg mass : 2037.8556 )
• Hex:6 HexNAc:5 dHex:1 (avg mass : 2152.9877 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:1 (avg mass : 2444.2456 )
• Hex:6 HexNAc:5 dHex:1 NeuAc:2 (avg mass : 2735.5035 )
• Hex:6 HexNAc:6 dHex:1 (avg mass : 2356.1827 )
• Hex:6 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2647.4406 )
• Hex:6 HexNAc:6 dHex:1 NeuAc:2 (avg mass : 2938.6985 )
• Hex:6 HexNAc:7 dHex:1 (avg mass : 2559.3777 )
• Hex:6 HexNAc:7 dHex:1 NeuAc:1 (avg mass : 2850.6356 )
• Hex:7 HexNAc:2 (avg mass : 1559.4021 )
• Hex:7 HexNAc:6 dHex:1 (avg mass : 2518.3251 )
• Hex:7 HexNAc:6 dHex:1 NeuAc:1 (avg mass : 2809.583 )
• Hex:7 HexNAc:6 dHex:1 NeuAc:2 (avg mass : 3100.8409 )
• Hex:7 HexNAc:6 dHex:1 NeuAc:3 (avg mass : 3392.0988 )
• Hex:7 HexNAc:7 dHex:1 (avg mass : 2721.5201 )
• Hex:7 HexNAc:7 dHex:1 NeuAc:1 (avg mass : 3012.778 )
• Hex:7 HexNAc:7 dHex:1 NeuAc:2 (avg mass : 3304.0359 )
• Hex:8 HexNAc:2 (avg mass : 1721.5445 )
• Hex:9 HexNAc:2 (avg mass : 1883.6869 )

Disease

Reference

• A single glycan on IgE is indispensable for initiation of anaphylaxis (2015 - Kai-Ting C. Shade, Barbara Platzer, Nathaniel Washburn, Vinidhra Mani, Yannic C. Bartsch, Michelle Conroy, Jose D. Pagan, Carlos Bosques, Thorsten R. Mempel, Edda Fiebiger, Robert M. Anthony) / Status : Reviewed
• Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS (2014 - Chen R, Seebun D, Ye M, Zou H, Figeys D) / Status : Reviewed

Reported glycosite

• Adipocyte plasma membrane-associated protein / Homo sapiens
  •        Asn-160
• Immunoglobulin epsilon chain c region / Homo sapiens
  •        Asn-21
  •        Asn-49
  •        Asn-99
  •        Asn-146
  •        Asn-252
  •        Asn-264
  •        Asn-275

Mass spectrometry observed peptide